Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development
Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development
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DOI：
10.1101/gad.1836009
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发表时间：
2009-10-15
影响因子：
10.5
通讯作者：
Weissman, Irving L.
中科院分区：
文献类型：
--
作者：
Inlay, Matthew A.;Bhattacharya, Deepta;Weissman, Irving L.
常见的淋巴样祖细胞（CLP）克隆产生B细胞和T细胞谱系，但在体内几乎没有髓样潜能。然而，一些研究声称，上游淋巴启动多能祖细胞（LMPP）是胸腺播种人口，并建议CLPs主要是B细胞限制。为了鉴定区分功能性CLP与B细胞祖细胞的表面蛋白，我们使用了一种新的计算方法，即挖掘发育调节基因（MiDReG）。我们鉴定了Ly6d，其将CLP分成两个不同的群体：一个保留了完整的体内淋巴潜能，并且在早期时间点比LMPP产生更多的胸腺细胞，另一个基本上表现为B细胞祖细胞。
Common lymphoid progenitors (CLPs) clonally produce both B-and T-cell lineages, but have little myeloid potential in vivo. However, some studies claim that the upstream lymphoid-primed multipotent progenitor (LMPP) is the thymic seeding population, and suggest that CLPs are primarily B-cell-restricted. To identify surface proteins that distinguish functional CLPs from B-cell progenitors, we used a new computational method of Mining Developmentally Regulated Genes (MiDReG). We identified Ly6d, which divides CLPs into two distinct populations: one that retains full in vivo lymphoid potential and produces more thymocytes at early time-points than LMPP, and another that behaves essentially as a B-cell progenitor.