Short-term environmental enrichment enhances synaptic plasticity in hippocampal slices from aged rats.
Short-term environmental enrichment enhances synaptic plasticity in hippocampal slices from aged rats.
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DOI：
10.1016/j.neuroscience.2016.05.020
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发表时间：
2016-08-04
期刊：
影响因子：
3.3
通讯作者：
Izumi Y
中科院分区：
文献类型：
--
作者：
Stein LR;O'Dell KA;Funatsu M;Zorumski CF;Izumi Y
认知中的神经元相关变化反映在记忆和学习的细胞模型中的损伤，例如长时程增强（LTP）和长时程抑郁（LTD）。在年轻的啮齿动物中，环境丰富（EE）可以增强记忆，改变LTP和LTD，以及逆转衰老引起的认知缺陷。短期EE是否有益于老年啮齿动物的认知和突触可塑性尚不清楚。在这里，我们测试，如果短期EE可以克服与年龄相关的损伤诱导LTP和LTD。LTP和LTD不能诱导在海马脑片的CA 1区的控制，老年大鼠使用标准的刺激，是非常有效的年轻大鼠。然而，老年同窝仔暴露于EE三周，使成功诱导LTP和LTD。EE促进LTP依赖于N-甲基-D-天冬氨酸受体（NMDAR）。这些突触可塑性的改变发生在磷酸化cAMP反应元件结合蛋白和血管内皮生长因子水平升高，但在其他几个突触和细胞标志物的变化的情况下。重要的是，我们的研究表明，即使是相对较短的EE时间也足以改变老年动物的突触可塑性和与认知功能相关的分子标记物。
Age-associated changes in cognition are mirrored by impairments in cellular models of memory and learning, such as long-term potentiation (LTP) and long-term depression (LTD). In young rodents, environmental enrichment (EE) can enhance memory, alter LTP and LTD, as well as reverse cognitive deficits induced by aging. Whether short-term EE can benefit cognition and synaptic plasticity in aged rodents is unclear. Here, we tested if short-term EE could overcome age-associated impairments in induction of LTP and LTD. LTP and LTD could not be induced in the CA1 region of hippocampal slices in control, aged rats using standard stimuli that are highly effective in young rats. However, exposure of aged littermates to EE for three weeks enabled successful induction of LTP and LTD. EE-facilitated LTP was dependent upon N-methyl-D-aspartate receptors (NMDARs). These alterations in synaptic plasticity occurred with elevated levels of phosphorylated cAMP response element-binding protein and vascular endothelial growth factor, but in the absence of changes in several other synaptic and cellular markers. Importantly, our study suggests that even a relatively short period of EE is sufficient to alter synaptic plasticity and molecular markers linked to cognitive function in aged animals.