Nanospray desorption electrospray ionization: an ambient method for liquid-extraction surface sampling in mass spectrometry
Nanospray desorption electrospray ionization: an ambient method for liquid-extraction surface sampling in mass spectrometry
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DOI：
10.1039/c0an00312c
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Laskin, Alexander
中科院分区：
文献类型：
--
作者：
Roach, Patrick J.;Laskin, Julia;Laskin, Alexander
纳米喷雾解吸电喷雾电离（nano-DESI）质谱是一种常压液体萃取-电离技术，用于分析基质上的有机和生物分子。分析物被解吸到两个毛细管和分析表面之间形成的溶剂桥中。一根毛细管提供溶剂以产生和维持桥，而第二根毛细管将溶解的分析物从桥输送到质谱仪。在质谱仪的入口和主毛细管之间施加高电压产生自抽吸纳米喷雾。这种方法能够分离解吸和电离事件，从而提供对分析物的解吸、电离和传输的独立控制。我们提出的方法的分析能力，并讨论其潜在的成像应用。
Nanospray desorption electrospray ionization (nano-DESI) mass spectrometry is presented as an ambient pressure liquid extraction-ionization technique for analysis of organic and biological molecules on substrates. Analyte is desorbed into a solvent bridge formed between two capillaries and the analysis surface. One capillary supplies solvent to create and maintain the bridge, while the second capillary transports the dissolved analyte from the bridge to the mass spectrometer. A high voltage applied between the inlet of mass spectrometer and the primary capillary creates a self-aspirating nanospray. This approach enables the separation of desorption and ionization events, thus providing independent control of desorption, ionization, and transport of the analyte. We present analytical capabilities of the method and discuss its potential for imaging applications.