Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing
Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing
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DOI：
10.1021/ja047252a
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发表时间：
2004-10-27
影响因子：
15
通讯作者：
Kraemer, R
中科院分区：
文献类型：
--
作者：
Brunner, J;Kraemer, R
通过将荧光素连接到DNA的3 '-末端并将铜（II）络合物连接到DNA的5'-末端来制备铜（II）-淬灭的分子信标。在互补DNA的存在下，铜（II）和染料在空间上分开的双链体和荧光增加高达15倍，具有良好的歧视单碱基错配。
A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3‘-end and a copper(II) complex to the 5‘-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.