A biodegradable composite scaffold for cell transplantation
A biodegradable composite scaffold for cell transplantation
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DOI：
10.1016/s0736-0266(01)00074-2
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发表时间：
2002-01-01
影响因子：
2.8
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Ameer, GA;Mahmood, TA;Langer, R
细胞移植正在迅速成为治疗疾病和损伤的一种治疗选择。然而，在非织造聚合物网或凝胶中种植细胞的标准技术可能不适合立即植入或手术操作新鲜分离的细胞。因此，开发了一种可生物降解的复合系统，作为一种将细胞快速包裹在预定形状的载体内的方法，以潜在地促进细胞输送到靶部位(例如，无血管区的半月板撕裂)。该复合结构由新鲜分离的细胞组成，在这种情况下是猪软骨细胞，被包裹在纤维蛋白凝胶相中，并分散在聚乙醇酸(PGA)无纺布的空隙体积中。复合材料培养长达4周。纤维蛋白凝胶的体外降解是通过凝胶包裹的尿激酶法进行的。培养28d时，复合支架的单位细胞糖胺多聚糖(GAG)含量是单纯PGA细胞构建组的2.6倍，是天然猪软骨的88%。复合支架中每细胞的总胶原含量与PGA细胞构建组(P&gT；0.02)没有显著差异，代表了天然软骨测定值的40%。改变包埋尿激酶的浓度可以控制纤维蛋白凝胶的降解。(C)2002年骨科研究会。爱思唯尔科学有限公司出版。版权所有。
Cell transplantation is rapidly becoming a therapeutic option to treat disease and injury. However standard techniques for cell seeding on non-woven polymer meshes or within gels may not be suitable for immediate implantation or surgical manipulations of freshly isolated cells. Therefore, a biodegradable composite system was developed as a way to rapidly entrap cells within a support of predefined shape to potentially facilitate cell delivers into a target site (e.g. meniscal tears in the avascular zone). The composite construct consisted of freshly isolated cells, in this case pig chondrocytes, entrapped in a fibrin gel phase and dispersed throughout the void volume of a polyglycolic acid (PGA) non-woven mesh. Composites were cultured for up to 4 weeks. In vitro degradation of fibrin gel was evaluated via gel-entrapped urokinase. At 28 days in culture, glycosaminoglycan (GAG) content per cell in the composite scaffolds was 2.6 times that of the PGA-only cell construct group and 88% that of native pig cartilage. Total collagen content per cell in the composite scaffolds was not significantly different from the PGA-only cell construct group (P > 0.02) and represented 40% of the value determined for native cartilage. Varying the concentration of entrapped urokinase could effect controlled degradation of fibrin gel. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.