Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction
Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction
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DOI：
10.1002/btpr.383
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发表时间：
2010-05
影响因子：
2.9
通讯作者：
K. Nandini;N. Rastogi
中科院分区：
文献类型：
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作者：
K. Nandini;N. Rastogi
使用单步反胶束辅助提取研究了从乳清中提取乳过氧化物酶 (EC 1.11.1.7)，并与反胶束提取进行了比较。反胶束辅助萃取导致污染蛋白质的萃取和水相中乳过氧化物酶的回收，从而实现其纯化。经过正向和反向步骤后，在优化条件下进行反胶束萃取，乳过氧化物酶的活性恢复了86.60%，纯化系数分别达到了86.60%和3.25倍。而反胶束辅助提取导致乳过氧化物酶的活性回收率更高（127.35%）和纯化因子（3.39 倍）。十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱也证明，与反胶束提取的乳过氧化物酶相比，反胶束辅助提取获得了更高的纯化效果。 © 2010 美国化学工程师生物技术研究所。计划，2010
The extraction of lactoperoxidase (EC 1.11.1.7) from whey was studied using single step reverse micelles‐assisted extraction and compared with reverse micellar extraction. The reverse micelles‐assisted extraction resulted in extraction of contaminating proteins and recovery of lactoperoxidase in the aqueous phase leading to its purification. Reverse micellar extraction at the optimized condition after forward and backward steps resulted in activity recovery of lactoperoxidase and purification factor of the order of 86.60% and 3.25‐fold, respectively. Whereas reverse micelles‐assisted extraction resulted in higher activity recovery of lactoperoxidase (127.35%) and purification factor (3.39‐fold). The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) profiles also evidenced that higher purification was obtained in reverse micelles‐assisted extraction as compared of reverse micellar extracted lactoperoxidase. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010