A GENE INDUCIBLE BY SERUM GROWTH-FACTORS ENCODES A MEMBER OF THE STEROID AND THYROID-HORMONE RECEPTOR SUPERFAMILY
A GENE INDUCIBLE BY SERUM GROWTH-FACTORS ENCODES A MEMBER OF THE STEROID AND THYROID-HORMONE RECEPTOR SUPERFAMILY
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DOI：
10.1073/pnas.85.22.8444
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
LAU, LF
中科院分区：
文献类型：
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作者：
HAZEL, TG;NATHANS, D;LAU, LF
我们之前通过cDNA克隆鉴定了一组在小鼠成纤维细胞G0/G1过渡（细胞周期再进入）期间表达的基因。这些直接早期基因在加入血清或纯化生长因子的几分钟内被转录激活，并且它们的mrna在蛋白质合成抑制剂的存在下被超诱导。我们在这里报告了其中一个基因，由nur/77 cDNA（最初称为3CH77）代表，编码配体结合转录因子超家族的成员，包括类固醇和甲状腺激素受体。nur/77 cDNA序列编码一个含有601个氨基酸的蛋白，其中包含两个与该核受体超家族成员相似的序列区域，对应于它们的dna结合域和配体结合域。这些结果表明，生长因子诱导的即时早期基因nur/77编码一种配体结合蛋白，调节对生长因子的基因组反应。
We previously identified, by cDNA cloning, a set of genes that are expressed during the G0/G1 transition (cell cycle reentry) in mouse fibroblasts. These immediate early genes are transcriptionally activated within minutes of addition of serum or purified growth factors, and their mRNAs are superinduced in the presence of protein-synthesis inhibitors. We report here that one of these genes, represented by nur/77 cDNA (originally called 3CH77), encodes a member of the superfamily of ligand-binding transcription factors that includes the steroid and thyroid hormone receptors. The nur/77 cDNA sequence encodes a protein of 601 amino acids containing two regions of sequence similarity to members of this nuclear receptor superfamily, corresponding to their DNA-binding and ligand-binding domains. These results suggest that the growth factor-inducible immediate early gene nur/77 encodes a ligand-binding protein that regulates the genomic response to growth factors.