Expression of multiple thyroid hormone receptor mRNAs in human oocytes, cumulus cells, and granulosa cells.
Expression of multiple thyroid hormone receptor mRNAs in human oocytes, cumulus cells, and granulosa cells.
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DOI：
10.1093/molehr/3.7.555
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发表时间：
1997-07
影响因子：
4
通讯作者：
Sue S. Zhang;Alberto J. Carrillo;Douglas S. Darling
中科院分区：
文献类型：
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作者：
Sue S. Zhang;Alberto J. Carrillo;Douglas S. Darling
甲状腺激素对卵巢功能有不同的影响。我们检测了三种人类卵泡细胞中甲状腺激素受体（TR） mrna（包括trα -1、trβ -1、trβ -2和c- erbaα -2亚型）的表达，并测定了人类卵泡液中游离三碘甲状腺原氨酸（T3）的浓度。采用逆转录聚合酶链式反应（RT-PCR）和Southern blot分析方法检测来自Alliant医院生育中心体外受精（IVF）患者的人受精失败卵母细胞、颗粒（GC）和积云（CC）细胞的TR mRNA表达。还分析了人类精子，以确定用CC获得的结果是否会受到精子存在的影响。在每种细胞类型中扩增β - actin mRNA作为RT-PCR的阳性对照。我们的研究结果表明，人卵母细胞表达trα -1、trβ -1、trβ -2和c- erbaα -2 mrna，这些相同的亚型在人颗粒细胞和积云细胞中都有表达。将GC与CC进行比较，RT-PCR产物的表观数量没有差异，表明这些rna的表达模式相似。精子中检测到β -肌动蛋白mRNA，但未检测到trα -1表达。在卵泡液中测量的游离T3浓度与甲状腺功能正常患者的血清浓度相似或略低。这些数据表明，在人卵母细胞中有几种TR mRNA的亚型表达，因此甲状腺激素可能对卵母细胞有直接影响，也可能对GC和CC有影响，此外甲状腺激素可能通过CC对卵母细胞有间接影响。
Thyroid hormones have diverse effects on ovarian function. We examined the expression of thyroid hormone receptor (TR) mRNAs (including TRalpha-1, TRbeta-1, TRbeta-2, and c-erbAalpha-2 isoforms) in three types of cells from human follicles, and determined the concentration of free tri-iodothyronine (T3) present in human follicular fluid. Human failed-fertilized oocytes, granulosa (GC) and cumulus (CC) cells from patients of the in-vitro fertilization (IVF) programme at Alliant Hospital Fertility Center were used to detect TR mRNA expression using reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. Human spermatozoa were also analysed to determine whether results obtained with CC would be affected by the presence of spermatozoa. beta-Actin mRNA was amplified in each cell type as a positive control for the RT-PCR. Our results show that human oocytes express TRalpha-1, TRbeta-1, TRbeta-2, and c-erbAalpha-2 mRNAs and that these same isoforms are expressed in both human granulosa cells and cumulus cells. No differences were detected in the apparent amounts of RT-PCR products when comparing GC with CC, suggesting a similar pattern of expression of these RNAs. beta-actin mRNA was detected in spermatozoa, but TRalpha-1 expression was not detectable. The concentrations of free T3 measured in follicular fluid were similar to, or slightly below, those in serum of euthyroid patients. These data demonstrated that several isoforms of TR mRNA are expressed in the human oocyte, and hence thyroid hormone may have direct affects on the oocyte, as well as on GC and CC. In addition thyroid hormone may have indirect effects on the oocytes via the CC.