Performance and population analysis of hydrogen production from sugarcane juice by non-sterile continuous stirred tank reactor augmented with Clostridium butyricum
Performance and population analysis of hydrogen production from sugarcane juice by non-sterile continuous stirred tank reactor augmented with Clostridium butyricum
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DOI：
10.1016/j.ijhydene.2010.05.120
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发表时间：
2011-07
影响因子：
7.2
通讯作者：
Sakchai Pattra;C. Lay;Chiu-Yue Lin;S. O-thong;A. Reungsang
中科院分区：
文献类型：
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作者：
Sakchai Pattra;C. Lay;Chiu-Yue Lin;S. O-thong;A. Reungsang
以甘蔗汁为原料，研究了丁酸梭菌强化连续搅拌反应器（CSTR）在不同水力停留时间（HRT）下的非无菌运行。在HRT为4 h时，最大产氢速率和产氢率分别为3.38mmolH2/L/h和1.0molH2/mol己糖。采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）技术分析了非无菌条件下发酵体系中强化微生物与正常植物群的关系。最初，在HRT 36小时，其他物种相关的哈尔滨乳杆菌和肺炎克雷伯氏菌作为一个主要群体在反应器中。当HRT降至12、6和4 h时，C.丁酸菌存在竞争，哈尔滨乳杆菌与克氏杆菌之间存在竞争。肺炎。结果表明，C.在正常植物群（K.肺炎）。
Non-sterile operation of continuous stirred tank reactor (CSTR) augmented with Clostridium butyricum and fed with sugarcane juice was studied at various hydraulic retention time (HRT). The maximum hydrogen production rate and yield of 3.38 mmol H2/L/h and 1.0 mol H2/mol hexose consumed, respectively, were achieved at HRT 4 h. The relationship of the augmented microorganism and normal flora in the fermentation system under non-sterile condition were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Initially, at HRT 36 h, other species related to Lactobacillus harbinensis and Klebseilla pneumoniae were present as a major group in the reactor. When HRT was decreased to 12, 6 and 4 h, C. butyricum was present with a competition between L. harbinensis and K. pneumoniae. Results indicated that augmented C. butyricum could compete with contaminated microorganisms during non-sterile operation at low HRT (12–4 h) with the support of normal flora (K. pneumoniae).