Three-dimensional electron paramagnetic resonance imaging of mice using ascorbic acid sensitive nitroxide imaging probes
Three-dimensional electron paramagnetic resonance imaging of mice using ascorbic acid sensitive nitroxide imaging probes
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使用抗坏血酸敏感硝基氧成像探针对小鼠进行三维电子顺磁共振成像
DOI：
10.1080/10715762.2021.1991918
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发表时间：
2021
影响因子：
3.3
通讯作者：
Fujii Hirotada G.
中科院分区：
文献类型：
--
作者：
Sato-Akaba Hideo;Emoto Miho C.;Yamada Ken-ichi;Koshino Hisashi;Fujii Hirotada G.
氮氧化物化合物已被电子顺磁共振(EPR)用作氧化还原敏感的成像探针，用于评估体内的氧化应激。氮氧化物的快速氧化还原反应有利于评估更高的氧化还原灵敏度；然而，由于各种氮氧化物的活体还原速度非常快，无法在现有EPR成像仪的缓慢操作速度下捕捉到，因此尚未作为成像探针进行试验。为了克服这一限制，我们改进了EPR系统，以提供稳定和高灵敏度的成像操作。我们要求改进的电子顺磁共振成像仪使用两种有用的氮氧化物成像探针--4-羟基-2，2，6，6-四甲基哌啶1-氧基(Tempol)和2，6-dispiro-4′，4ʺ-dipyrane-piperidine-4-one-N-oxyl(DiPy)对小鼠脑进行三维(3D)EPR成像。DiPy与抗坏血酸的二级反应速率常数是Tempoll的10倍。改进后的EPR成像仪获得了清晰的小鼠脑的3D EPR图像，证明了Tempoll可以以未配对的电子存在。该成像仪还成功地获得了小鼠头部注射DiPy后的3D EPR图像。由于可以在6 的时间内获得126个投影，因此，3DEPR成像可以直观地显示DiPy进入大脑、在脑内分布和在脑内被还原的顺序过程。EPR成像仪的这些改进将使以前因快速减少而不适合作为成像探针的有用的氮氧化物成像探针被考虑用于体内系统中灵敏的氧化还原评估。
Nitroxide compounds have been used as redox-sensitive imaging probes by electron paramagnetic resonance (EPR) for assessing oxidative stressin vivo. Fast redox reactions of nitroxide radicals are favorable for assessment of higher redox sensitivity; however, a variety of nitroxides have not been trialed for use as imaging probes due to their very rapidin vivoreduction, which cannot be captured at the slow operation speed of existing EPR imagers. To overcome this limitation, we improved our EPR system to provide a stable and highly sensitive imaging operation. We challenged the improved EPR imager to perform three-dimensional (3D) EPR imaging of mouse brain using two useful nitroxide imaging probes, 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) and 2,6-dispiro-4′,4ʺ-dipyrane-piperidine-4-one-N-oxyl (DiPy). The second-order rate constant of DiPy with ascorbic acid is 10 times larger than that of Tempol. The improved EPR imager obtained clear 3D EPR images of mouse brain and demonstrated that Tempol could exist with an unpaired electron. The imager also successfully obtained 3D EPR images of mouse head after administration of DiPy. As 126 projections can be acquired in a period of 6 s, 3D EPR imaging can visualize the sequential process of DiPy entering the brain, being distributed within the brain, and being reduced within the brain. These improvements to the EPR imager will enable useful nitroxide imaging probes that were previously unsuitable as imaging probes due to their rapid reduction to be considered for use for sensitive redox assessment in anin vivosystem.
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使用两种类型的血脑屏障可渗透的硝基氧自由基对大鼠大脑进行体内时间 EPR 成像。
影响因子：
2.5
作者：
H. Yokoyama;O. Itoh;M. Aoyama;H. Obara;H. Ohya;H. Kamada
通讯作者：
H. Kamada
Kazunori Anzai：“通过与放射自显影比较来评估 ESR-CT 成像，以评估血脑屏障渗透性旋转探针 MC-PROXYL 在啮齿动物大脑中的分布”Magn.Reson.Imaging。
DOI：
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发表时间：
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影响因子：
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作者：
通讯作者：
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使用 Elevtron 自旋共振 (ESR) 成像和体内 L 波段 ESR 评估自发性高血压大鼠脑中的氧化应激。
DOI：
--
发表时间：
2004
期刊：
Hypertens Res. 27
影响因子：
--
作者：
M-C Lee;H.Shoji;K.Anzai;N.Ikota;T.Oawa;et al.
通讯作者：
et al.
影响因子：
--
作者：
Basso, Luis G Mansor;Mendes, Luis F Santos;Costa-Filho, Antonio J
通讯作者：
Costa-Filho, Antonio J