Regional analysis of the ependyma of the third ventricle of rat by light and electron microscopy
Regional analysis of the ependyma of the third ventricle of rat by light and electron microscopy
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DOI：
10.1111/j.1439-0264.2007.00786.x
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发表时间：
2008-02-01
影响因子：
0.9
通讯作者：
Mathew, T. C.
中科院分区：
文献类型：
--
作者：
Mathew, T. C.
脑室的室管膜位于脑室腔和脑实质的交界处。室管膜细胞参与脑内的各种功能，并在脑脊液化学原理的产生中发挥重要作用。用光镜和电子显微镜方法对成年大鼠第三脑室室管膜和室管膜下的局部变异进行了组织学研究。对于光镜分析，使用了甲基丙烯酸酯切片。除常规苏木精-伊红(H和E)染色用于组织学研究外，还用甲苯胺蓝、甲酚紫和高碘酸Schiff试剂(PAS)进行染色。对第三脑室室管膜的区域性分析显示，在大多数区域室管膜是单层的。第三脑室腹侧部的侧壁和底板呈多层室管膜。为了在光镜水平上描述，室管膜细胞根据细胞形状(扁平、立方或柱状)、纤毛的存在或不存在以及细胞中存在的细胞质颗粒的数量来分类。透射电子显微镜研究表明，这些颗粒代表室管膜的细胞器。室管膜下也表现出区域性的形态变化，在大多数情况下，含有神经胶质和神经元成分。在特定的脑核区域，神经元是室管膜下的主要细胞类型。PAS染色未见室管膜胞浆内有阳性颗粒。室管膜上的特征性成分存在于第三脑室的室管膜表面。
Ependymal lining of cerebral ventricles lies at the interface between the ventricular cavities and the brain parenchyma. Ependymal cells are involved in various functions within the brain and play a major role in the production of the chemical principals of the cerebrospinal fluid. Histological studies on the regional variation of the third ventricular ependyma and the subependyma of adult rats were carried out by light and electron microscopic methods. For light microscopic analysis, methacrylate sections were used. In addition to the routine haematoxylin and eosin (H and E) staining for histological Studies, the sections were stained with toluidine blue, cresyl violet and periodic acid Schiffs reagent (PAS). A regional analysis of the ependyma of the third ventricle showed that in most regions the ependyma was monolayered. The sidewalls and floor of the ventral portion of the third ventricle showed a multilayered ependyma. For descriptive purposes at the light microscopic level, the ependymal cells were classified, based on the cell shape (flat, cuboidal or columnar), presence or absence of cilia and the number of cytoplasmic granules present in the cells. Studies of transmission electron microscope have shown that these granules represent the cell organelles of the ependyma. The subependyma also showed a regional morphological variation, and, in most instances, contained glial and neuronal elements. In regions of specific brain nuclei, neurons were the major cell type of the subependyma. PAS staining did not show any positive granules in the ependymal cytosol. Characteristic supraependymal elements were present at the ependymal surface of the third ventricle.