A menstrual cycle pattern for cytokine levels exists in HIV-positive women: implication for HIV vaginal and plasma shedding
A menstrual cycle pattern for cytokine levels exists in HIV-positive women: implication for HIV vaginal and plasma shedding
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DOI：
10.1097/00002030-200108170-00011
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发表时间：
2001-08-17
期刊：
影响因子：
3.8
通讯作者：
Landay, A
中科院分区：
文献类型：
--
作者：
Al-Harthi, L;Kovacs, A;Landay, A
目的：评估hiv阳性妇女月经周期对血浆和生殖器细胞因子水平的影响，阴道和血浆细胞因子、CD4和CD8 T细胞波动以及生殖器和血浆病毒载量之间的相互关系。方法：对55例CD4细胞计数< 350个/个的hiv阳性妇女进行月经周期血浆和宫颈阴道灌洗液标本采集。采用酶联免疫吸附法检测样品的IL-1 β、IL-6、IL-4、IL-8、IL-10、TGF β、TNF α、INF γ、MIP1 α、MIP1 β、RANTES和TNFR-II。流式细胞术检测CD4和CD8 T细胞的表达。使用重复测量回归模型来评估月经周期对细胞因子和病毒载量的影响。使用多变量重复回归模型来评估所选细胞因子之间以及所选细胞因子与HIV病毒载量之间的相关性。结果：阴道IL-1 β、IL-4、IL-6、IL-8、IL-10、MIP1 β、RANTES、TGF β、TNFR-II在月经期间显著升高，其他时期无明显变化。血浆细胞因子水平在月经周期中没有改变。阳性念珠菌培养增加月经期间阴道IL-8，而阴道分泌物与阴道IL-4、IL-10和RANTES的减少有关。CD4和CD8细胞数量不随月经周期变化。阴道细胞因子水平仅与阴道病毒载量相关，以采样方法依赖的方式。结论：我们提供了月经期间阴道细胞因子水平升高的证据，它似乎调节阴道而不是血浆HIV的脱落，这表明HIV阳性妇女的月经周期模式存在细胞因子的产生影响HIV的阴道脱落。(C) 2001 Lippincott Williams & Wilkins。
Objectives: To evaluate the effect of the menstrual cycle in HIV-positive women on plasma and genital cytokine levels, interrelationships between vaginal and plasma cytokines, CD4 and CD8 T cell fluctuations, and genital and plasma viral loads.Methods: Plasma and cervicovaginal lavage specimens were collected from 55 HIV-positive women with CD4 cell counts < 350 cells/mul during phases of the menstrual cycle. Samples were assayed for IL-1 beta, IL-6, IL-4, IL-8, IL-10, TGF beta, TNF alpha, INF gamma, MIP1 alpha, MIP1 beta, RANTES, and TNFR-II using enzyme-linked immunosorbent assays. CD4 and CD8 T cell expression was evaluated by flow cytometry. Repeated measures regression models were used to assess the effect of the menstrual cycle on cytokines and viral load. Multivariate repeated regression models were used to assess the correlation among selected cytokines and between selected cytokines and HIV viral load.Results: Vaginal IL-1 beta, IL-4, IL-6, IL-8, IL-10, MIP1 beta, RANTES, TGF beta, and TNFR-II were significantly elevated during menses but were not altered during other phases. Plasma cytokine levels were not altered during the menstrual cycle. A positive Candida culture increased vaginal IL-8 during menses, whereas vaginal discharge was associated with a reduction in vaginal IL-4, IL-10, and RANTES. CD4 and CD8 cell numbers did not vary with the menstrual cycle. Vaginal cytokine levels correlated only with vaginal viral load, in a sampling method-dependent manner.Conclusion: We provide evidence of elevated vaginal cytokine levels during menses, which appear to regulate vaginal and not plasma HIV shedding, suggesting that a menstrual cycle pattern exists for cytokine production in HIV-positive women impacting vaginal shedding of HIV. (C) 2001 Lippincott Williams & Wilkins.