Histomorphologic characterization of noncarious and caries-affected dentin/adhesive interfaces.
Histomorphologic characterization of noncarious and caries-affected dentin/adhesive interfaces.
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DOI：
10.1111/j.1532-849x.2006.00079.x
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发表时间：
2006-03-01
期刊：
影响因子：
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通讯作者：
Spencer, Paulette
中科院分区：
文献类型：
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作者：
Haj-Ali, Reem;Walker, Mary;Spencer, Paulette
目的：本研究的目的是比较粘合到非龋齿和受龋齿影响的牙本质时的牙本质/粘合剂界面特征。材料和方法：将七颗拔出的、未萌出的第三磨牙切成两半。在每颗牙齿的一半上制作人工龋齿，将另一半作为对照。根据制造商的湿粘合技术说明并在口腔中的环境条件下使用 UNO 粘合剂处理牙本质表面。每颗半牙的牙本质/粘合界面部分用经典的骨染色剂戈德纳三色染色，并使用光学显微镜检查。直接从显微照片中测量暴露的胶原蛋白的宽度，并对粘合剂渗透进行定性分析。结果：粘合剂封装脱矿牙本质基质的程度和范围反映在染色切片的色差上，未龋齿牙本质切片显示出胶原蛋白封装程度优于受龋齿影响的牙本质切片。受龋齿影响的牙本质/粘合剂界面暴露的胶原蛋白的总体平均宽度显着更大 (p < 或 = .05)，为 8.6 (1.7) 微米，而与非龋齿牙本质/粘合剂界面的暴露胶原蛋白的总体平均宽度 6.0 (1.5) 微米相比。结论：受龋齿影响的牙本质/界面的形态特征表明，暴露的胶原蛋白区域有所增加，并且 与非龋牙本质相比，粘合剂渗透的质量下降。证据表明牙本质基质特性对牙本质/粘合剂界面结构有显着影响。
PURPOSE: The purpose of this study was to compare the dentin/adhesive interfacial characteristics when bonding to noncarious as well as caries-affected dentin.MATERIALS AND METHODS: Seven extracted, unerupted, third molars were sectioned into halves. Artificial caries was created on one-half of each tooth, leaving the other half as a control. Dentin surfaces were treated with UNO adhesive according to the manufacturer's instructions for the wet-bonding technique and under environmental conditions present in the oral cavity. Dentin/adhesive interface sections of each half-tooth were stained with Goldner's trichrome, a classic bone stain, and examined using light microscopy. The width of exposed collagen was measured directly from photomicrographs, and adhesive penetration was analyzed qualitatively.RESULTS: The degree and extent to which the adhesive encapsulated the demineralized dentin matrix were reflected in the color difference in the stained sections with the noncarious dentin sections showing a degree of collagen encapsulation superior to that of the caries-affected dentin sections. The overall mean widths of exposed collagen were significantly (p < or = .05) greater at the caries-affected dentin/adhesive interface, 8.6 (1.7) microm, as compared with those at the noncarious dentin/adhesive interface, 6.0 (1.5) microm.CONCLUSIONS: The morphologic characteristics of the caries-affected dentin/interface suggest an increase in the exposed collagen zone and a decrease in the quality of the adhesive infiltration when compared with noncarious dentin. The evidence suggests that dentin substrate characteristics have a significant effect on the dentin/adhesive interface structure.