Functional evaluation of T helper, T suppressor, and B lymphocytes in lethally irradiated rhesus monkeys injected with autologous bone marrow.
Functional evaluation of T helper, T suppressor, and B lymphocytes in lethally irradiated rhesus monkeys injected with autologous bone marrow.
复制标题
对注射自体骨髓的致死辐照恒河猴进行 T 辅助细胞、T 抑制细胞和 B 淋巴细胞的功能评估。
DOI：
10.1097/00007890-199206000-00027
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发表时间：
1992
期刊：
影响因子：
6.2
通讯作者：
Good,RA
中科院分区：
文献类型：
--
作者：
Gengozian,N;Moriarty,C;Good,RA
用自体骨髓处理致死性辐照的恒河猴，所述自体骨髓已经（1）未处理，即正常;（2）用针对恒河猴T淋巴细胞的单克隆抗体去除T淋巴细胞;（3）用大豆凝集素（SBA-级分）分级分离;或（4）用SBA分级分离并通过E-玫瑰花结进一步去除T细胞。有没有动物之间的血液重建的差异，但都表现出显着降低的T辅助/T抑制细胞的比例在移植后的第一个10个月和降低的能力，他们的外周血白细胞（PBL）产生IG刺激商陆有丝分裂原。PBL产生IG的能力低于正常水平，通过反向溶血空斑试验中的空斑形成细胞来测量，不能完全用TH/TS比值的改变来解释，但与TH、TS和B淋巴细胞的功能状态相关。从实验动物PBL分离的不同淋巴细胞亚群与适当亚群的正常细胞共培养，以获得PWM刺激时IG合成。用正常骨髓治疗的动物在5个月后显示TH细胞功能恢复，但它们的TS细胞表现出过度的抑制活性，并持续到移植后20个月。相比之下，那些接受治疗的骨髓（单克隆抗体加补体，或SBA）的动物表现出延迟（12个月或更长时间）恢复正常TH细胞功能和TS细胞表达抑制活性的正常水平的早期回报。由于SBA-部分的骨髓含有很少或没有TH细胞和T细胞耗竭的骨髓与mAb也删除这些细胞，它建议的PBL的不同淋巴细胞亚群的免疫恢复的动力学的影响TH细胞在骨髓接种的存在或不存在。
Lethally irradiated rhesus monkeys were treated with autologous bone marrow that had either been (1) non-treated, ie, normal;(2) depleted of T lymphocytes with a monoclonal antibody directed against rhesus T lymphocytes;(3) fractionated with the soybean agglutinin (SBA-fraction); or (4) fractionated with SBA and further depleted of T cells by E-rosetting. There was no difference in hematologic reconstitution among the animals, but all showed a marked lowering of the T helper/T suppressor ratio during the first 10 months posttransplant and reduced capability of their peripheral blood leukocytes (PBL) to produce Ig upon stimulation with pokeweed mitogen. This subnormal ability of PBL to produce Ig, as measured by plaque-forming cells in a reverse hemolytic plaque assay, was not explained entirely by the altered TH/TS ratio but was correlated with the functional status of the TH, TS, and B lymphocytes. Isolated populations of the different lymphocyte subsets from PBL of the experimental animals were cocultured with normal cells of the appropriate subset to obtain Ig synthesis when stimulated with PWM. Animals treated with normal bone marrow showed recovery of TH cell function after 5 months, but their TS cells showed excessive suppressor activity that persisted for 20 months posttransplant. In contrast, those animals receiving treated marrow (mAb plus complement, or SBA) showed a much-delayed (12 months or more) return to normal TH cell function and an earlier return of TS cells expressing a normal level of suppressor activity. Since the SBA-fraction of marrow contains very few or no TH cells and T cell depletion of marrow with mAb also removes these cells, it is suggested that the kinetics of immune recovery of the different lymphocyte subsets of PBL is influenced by the presence or absence of TH cells in the marrow inocula.