Expression of the WASP verprolin-homologues (WAVE members) in human breast cancer
Expression of the WASP verprolin-homologues (WAVE members) in human breast cancer
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DOI：
10.1159/000136157
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发表时间：
2007-01-01
期刊：
影响因子：
3.5
通讯作者：
Jiang, W. G.
中科院分区：
文献类型：
--
作者：
Fernando, H. S.;Davies, S. R.;Jiang, W. G.
背景：WASP家族蛋白在细胞运动所需的膜突起的形成中起着至关重要的作用。WAVE蛋白是一个重要的亚家族，在肌动蛋白聚合中也起着至关重要的作用，而肌动蛋白聚合对细胞迁移至关重要。然而，人们对这个亚家族在癌症中的临床意义知之甚少。我们首次报道了WAVE分子在蛋白质和mRNA水平上在人类乳腺癌中的表达。材料和方法：分析122例乳腺癌和32例正常乳腺组织中3种波分子在mRNA和蛋白水平的表达，并与患者的病理、临床资料和预后(120个月随访)进行相关性分析。结果：3种波分子在乳腺组织中均有表达。WAVE2转录本在所有乳腺肿瘤中均有高水平表达。WAVE2的过度表达见于结节阳性病例以及中、低分化肿瘤。此外，WAVE2的高水平表达与随访时的疾病死亡相关(p=0.02)。在WAVE1和WAVE3转录本与乳腺癌细胞之间没有发现明显的关联。版权所有(C)2008 S.Karger AG，巴塞尔。
Background: The WASP family proteins have been indicated to play a vital role in the formation of membrane protrusions required for cell locomotion. WAVE proteins are an important subfamily that also plays a crucial role in actin polymerisation, which is vital to cell migration. However, not much is known about the clinical significance of this subfamily in cancers. We report, for the first time, the expression of the WAVE molecules, at protein and mRNA levels, in human breast cancer. Materials and Methods: The expression of the 3 WAVE molecules at the mRNA and protein levels in a cohort of 122 human breast cancers and 32 normal breast tissues were analysed and correlated with the patients' pathological and clinical information as well as outcome (120 months follow-up). Results: All 3 WAVE molecules were detected in mammary tissues. WAVE2 transcripts were expressed in high levels in all breast tumours. Over-expression of WAVE2 was seen in node-positive cases as well as in moderately and poorly differentiated tumours. Also, high levels of WAVE2 expression were associated with death due to disease (p = 0.02) at follow-up. No distinct associations were found between the WAVE1 and WAVE3 transcripts and the breast cancer cells. Copyright (c) 2008 S. Karger AG, Basel.