Analysis of the genetic diversity among mandarins (Citrus spp.) using RAPD markers
Analysis of the genetic diversity among mandarins (Citrus spp.) using RAPD markers
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DOI：
10.1023/a:1018300900275
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发表时间：
1998-01-01
期刊：
影响因子：
1.9
通讯作者：
Pompeu, J
中科院分区：
文献类型：
--
作者：
Coletta, HD;Machado, MA;Pompeu, J
利用RAPD分子标记对10个种和7个杂种共35份柑橘种质资源进行了遗传相似性分析。1个八聚体引物和22个十聚体引物共扩增出109个RAPD标记，其中45个具有多态性。用Jaccard系数计算遗传相似性，用UPGMA法生成表型图。所获得的RAPD足以产生一些加入特定的标记，并将这些加入聚类成几个组，其中许多根据田中或韦伯的系统单位。柑橘类内的遗传相似性较高（G（J）= 0.77），表明栽培柑橘的遗传基础较窄。与其它柑桔属物种（Citron [C. medica L.]和Pummelo [C. grandis Osbeck]）低得多（最小G（J）= 0.27）。我们认为，柑橘组是一个单一的种，C。reticulata布兰科，由几个遗传上不同的个体和大量的杂种组成，而不是一些分类学研究所提出的大量种。
RAPD markers were used to evaluate genetic similarity among 35 mandarin accessions, including 10 species and 7 hybrids. One octamer and twenty-two decamer primers produced 109 RAPDs, 45 of which were polymorphic. Jaccard coefficient was used to calculate genetic similarity, and UPGMA to generate the phenogram. The RAPDs obtained were sufficient to generate some accession-specific markers, and to separate these accessions by clustering them into several groups, many of them according to Tanaka's or Webber's systematic units. The genetic similarity within the mandarin group is high (G(J) = 0.77), and suggests that cultivated mandarins have a narrow genetic base. The genetic similarity of mandarins to other true citrus species (Citron [C. medica L.] and Pummelo [C. grandis Osbeck]) was much lower (minimum G(J) = 0.27). We propose that the mandarin group is a single species, C. reticulata Blanco, composed of several genetically different individuals and a great number of hybrids, rather than a large number of species as proposed by some taxonomic studies.