Naturalization of the microbiota developmental trajectory of Cesarean-born neonates after vaginal seeding.
Naturalization of the microbiota developmental trajectory of Cesarean-born neonates after vaginal seeding.
复制标题
DOI：
10.1016/j.medj.2021.05.003
复制
发表时间：
2021-08-13
期刊：
影响因子：
--
通讯作者：
Dominguez-Bello MG
中科院分区：
文献类型：
--
作者：
Song SJ;Wang J;Martino C;Jiang L;Thompson WK;Shenhav L;McDonald D;Marotz C;Harris PR;Hernandez CD;Henderson N;Ackley E;Nardella D;Gillihan C;Montacuti V;Schweizer W;Jay M;Combellick J;Sun H;Garcia-Mantrana I;Gil Raga F;Collado MC;Rivera-Viñas JI;Campos-Rivera M;Ruiz-Calderon JF;Knight R;Dominguez-Bello MG
早期微生物群扰动与涉及免疫基础的疾病有关。剖腹产 (CS) 出生的婴儿与阴道出生的婴儿相比，微生物群发育发生了改变。在这里，我们提出了第一个统计动力纵向研究，以确定 CS 出生后恢复与母体阴道液体接触的效果。使用 16S rRNA 基因测序，我们追踪了 177 名婴儿在出生第一年的多个身体部位的微生物轨迹； 98 例经阴道分娩，79 例经剖腹产，其中 30 例在出生后立即用产妇阴道纱布擦拭。成分张量分解分析证实，接触 CS 出生的婴儿的微生物群轨迹与阴道出生的婴儿的微生物群轨迹更接近。有趣的是，与 HMP 研究中的非孕妇相比，出生当天母体阴道微生物组的大多数扩增子序列变异与其他母体部位共享。这项观察性研究的结果提示迫切需要随机临床试验来测试微生物恢复是否可以降低与 CS 出生相关的疾病风险增加及其潜在机制。此外，它还为母体阴道液的多能性质提供了证据，为新生儿身体部位提供先锋细菌定殖者。这是第一项研究表明，通过恢复出生时的微生物暴露，CS 出生的婴儿的微生物群可以长期自然化。 C&D、Emch 基金、CIFAR、智利 CONICYT 和 SOCIPE、挪威公共卫生研究所、Emerald 基金会、NIH、国家司法研究所、Janssen。剖腹产会改变婴儿微生物群的成熟。宋等人。研究表明，剖腹产婴儿出生后立即恢复母体微生物可以使其微生物群的发育轨迹自然化。在围产期，母体阴道液似乎具有多能性，可以为新生儿的身体部位提供先锋细菌定植菌。
Early microbiota perturbations are associated with disorders that involve immunological underpinnings. Cesarean section (CS)-born babies show altered microbiota development in relation to babies born vaginally. Here we present the first statistically powered longitudinal study to determine the effect of restoring exposure to maternal vaginal fluids after CS birth. Using 16S rRNA gene sequencing, we followed the microbial trajectories of multiple body sites in 177 babies over the first year of life; 98 were born vaginally and 79 were born by CS, of which 30 were swabbed with a maternal vaginal gauze right after birth. Compositional tensor factorization analysis confirmed that microbiota trajectories of exposed CS-born babies aligned closer to that of vaginally born babies. Interestingly, the majority of amplicon sequence variants from maternal vaginal microbiomes on the day of birth were shared with other maternal sites, in contrast to non-pregnant women from the HMP study. The results of this observational study prompt the urgent need of randomized clinical trials to test whether microbial restoration reduces the increased disease risk associated with CS birth, and the underlying mechanisms. Also, it provides evidence for the pluripotential nature of maternal vaginal fluids to provide pioneer bacterial colonizers for the newborn body sites. This is the first study showing long term naturalization of the microbiota of CS-born infants by restoring microbial exposure at birth. C&D, Emch Fund, CIFAR, Chilean CONICYT and SOCHIPE, Norwegian Institute of Public Health, the Emerald Foundation, NIH, National Institute of Justice, Janssen. C-section alters the maturation of the infant microbiota. Song et al. show that restoring maternal microbes immediately after birth in Cesarean-born infants naturalizes their microbiota developmental trajectory. Perinatally, the maternal vaginal fluids appear pluripotential to provide pioneer bacterial colonizers for the newborn body sites.