From the Mouths of Monkeys: Detection of Mycobacterium tuberculosis Complex DNA From Buccal Swabs of Synanthropic Macaques
From the Mouths of Monkeys: Detection of Mycobacterium tuberculosis Complex DNA From Buccal Swabs of Synanthropic Macaques
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DOI：
10.1002/ajp.22022
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发表时间：
2012-07-01
影响因子：
2.4
通讯作者：
Jones-Engel, Lisa
中科院分区：
文献类型：
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作者：
Wilbur, Alicia K.;Engel, Gregory A.;Jones-Engel, Lisa
虽然结核分枝杆菌复合体（MTBC）感染了三分之一的人类，但关于非人灵长类动物（NHP）中分枝杆菌感染的流行率知之甚少。世纪以来，结核病一直被认为是对NHP物种的严重传染性威胁。MTBC检测的进展为研究这种知之甚少的病原体在不同NHP人群中的影响开辟了新的可能性。在这里，我们报告的横截面研究的结果，使用描述良好的分子方法来检测一个核酸序列（IS 6110）独特的MTBC。样本采集集中在口腔，这是MTBC的假定传播途径。从四个亚洲国家和直布罗陀的11个物种的263只猕猴中采集口腔拭子。与人类接触的背景包括自由放养、宠物、表演猴、动物园和猴庙。从口腔拭子中分离DNA后，聚合酶链反应（PCR）扩增了84只（31.9%）猕猴的IS 6110。一般而言，在世界卫生组织报告人类感染MTBC流行率较高的国家，NHP中MTBC DNA的流行率较高。这是第一次在猕猴的嘴里展示MTBC DNA。需要进一步的研究来确定这一发现在个体和群体水平上的意义。口腔样本的PCR有望作为一种方法来阐明NHP中的分枝杆菌景观，特别是在人类MTBC高患病率地区蓬勃发展的猕猴。Am. J. Primatol. 74：676-686，2012. (C)2012 Wiley Periodicals，Inc.
Although the Mycobacterium tuberculosis complex (MTBC) infects a third of all humans, little is known regarding the prevalence of mycobacterial infection in nonhuman primates (NHP). For more than a century, tuberculosis has been regarded as a serious infectious threat to NHP species. Advances in the detection of MTBC open new possibilities for investigating the effects of this poorly understood pathogen in diverse populations of NHP. Here, we report results of a cross-sectional study using well-described molecular methods to detect a nucleic acid sequence (IS6110) unique to the MTBC. Sample collection was focused on the oral cavity, the presumed route of transmission of MTBC. Buccal swabs were collected from 263 macaques representing 11 species in four Asian countries and Gibraltar. Contexts of contact with humans included free ranging, pets, performing monkeys, zoos, and monkey temples. Following DNA isolation from buccal swabs, the polymerase chain reaction (PCR) amplified IS6110 from 84 (31.9%) of the macaques. In general, prevalence of MTBC DNA was higher among NHP in countries where the World Health Organization reports higher prevalence of humans infected with MTBC. This is the first demonstration of MTBC DNA in the mouths of macaques. Further research is needed to establish the significance of this finding at both the individual and population levels. PCR of buccal samples holds promise as a method to elucidate the mycobacterial landscape among NHP, particularly macaques that thrive in areas of high human MTBC prevalence. Am. J. Primatol. 74:676-686, 2012. (C) 2012 Wiley Periodicals, Inc.