Fluorescent proteins for single-molecule fluorescence applications
Fluorescent proteins for single-molecule fluorescence applications
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DOI：
10.1002/jbio.200710024
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Seefeldt, Britta;Kasper, Robert;Sauer, Markus
我们给出了荧光蛋白GFP、YFP、DsRed和mCherry （DsRed的一个新衍生物）的单分子荧光数据。集合和单分子荧光实验证明mCherry是一种理想的适合单分子应用的荧光团，具有高光稳定性和罕见的荧光强度波动。虽然mCherry在所研究的荧光蛋白中表现出最低的荧光量子产率，但其优越的光物理特性表明mCherry是单分子荧光实验的理想替代品。由于其光谱特性和1.46 ns的短荧光寿命，mCherry是其他现有荧光蛋白的补充，被推荐用于高精度目标分子的跟踪和定位，荧光共振能量转移（FRET），荧光寿命成像显微镜（FLIM）或多色应用。mCherry在单分子荧光成像条件下表现出较高的光稳定性和亮度。(C) 2008年WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications.[GRAPHICS]mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim