Preparation and Analysis of DNA
Preparation and Analysis of DNA
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DOI：
10.1002/0471142727.mb0200s98
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发表时间：
2002-05
影响因子：
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通讯作者：
D. Moore;D. Dowhan
中科院分区：
文献类型：
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作者：
D. Moore;D. Dowhan
引言从各种来源制备和分离纯DNA的能力是许多分子生物学方案中的重要步骤。事实上，从限制性内切酶和聚合酶链式反应（PCR）产物中分离基因组、质粒或DNA片段已成为几乎每个实验室的常见日常实践。因此，本章从纯化和浓缩DNA（单位）的一般方案开始
INTRODUCTION T he ability to prepare and isolate pure DNA from a variety of sources is an important step in many molecular biology protocols. Indeed, the isolation of genomic, plasmid, or DNA fragments from restriction digests and polymerase chain reaction (PCR) products has become a common everyday practice in almost every laboratory. This chapter therefore begins with general protocols for purifying and concentrating DNA (UNITS