Rapid determination of viral RNA sequences in mosquitoes collected in the field
Rapid determination of viral RNA sequences in mosquitoes collected in the field
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DOI:
10.1016/j.jviromet.2007.07.008
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发表时间:
2007-12-01
影响因子:
3.1
通讯作者:
Mizutani, Tetsuya
中科院分区:
文献类型:
--
作者:
Kihara, Yuki;Satho, Tomomitsu;Mizutani, Tetsuya
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. (c) 2007 Published by Elsevier B.V.