Rapid determination of viral RNA sequences in mosquitoes collected in the field

Rapid determination of viral RNA sequences in mosquitoes collected in the field
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DOI:
10.1016/j.jviromet.2007.07.008
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发表时间:
2007-12-01
影响因子:
3.1
通讯作者:
Mizutani, Tetsuya
Mizutani, Tetsuya
中科院分区:
医学4区
文献类型:
--
作者:
Kihara, Yuki;Satho, Tomomitsu;Mizutani, Tetsuya

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一种用于快速测定病毒RNA序列(RDV)的方法应用于埃及伊蚊的匀浆收集在泰国的登革热(登革出血热)流行的地区,使用蚊子细胞系C6/36。检测登革病毒4型和细胞融合剂病毒的核酸序列。该RDV方法有可能成为检测已知和新出现的未知蚊媒病毒的标准方法。(c)2007年由Elsevier B. V.出版。
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. (c) 2007 Published by Elsevier B.V.