Antibacterial and osteogenesis performances of LL37-loaded titania nanopores in vitro and in vivo
Antibacterial and osteogenesis performances of LL37-loaded titania nanopores in vitro and in vivo
复制标题
LL37负载二氧化钛纳米孔的体外和体内抗菌和成骨性能
DOI：
10.2147/ijn.s198583
复制
发表时间：
2019-01-01
影响因子：
8
通讯作者：
Liu, Jinsong
中科院分区：
文献类型：
--
作者：
Shen, Xinkun;Al-Baadani, Mohammed A.;Liu, Jinsong
背景：许多研究表明，纳米管（NT）的尺寸可以显著影响成骨细胞在钛基材料上的行为。但由于碳纳米管与基体结合强度较弱，极大地限制了其应用。目的：本研究的目的是比较NT和纳米孔（NP）涂层的稳定性，并通过在NP结构中负载LL 37肽来进一步制备抗菌钛基材料。方法：用划痕仪测试NT和NP层的粘附强度。采用CCK 8、碱性磷酸酶活性、矿化和聚合酶链反应等方法检测MC 3 T3-E1细胞在不同基质上的增殖和分化情况。用平板法测定NP和NP/LL 37的抗菌率。此外，使用未感染和感染的模型进一步评估体内NP和NP/LL 373周围的成骨。结果如下：划痕试验表明，由于纳米管具有连续的孔结构和较厚的管壁，因此纳米管层与基体的结合强度比独立的纳米管结构更强。体外细胞实验结果显示，NP组MC 3 T3-E1细胞的早期粘附、铺展和成骨分化能力均优于NT组。此外，基于多孔材料的药物储库特性，还使用NP基底负载抗菌LL 37肽。负载LL 37后，NP的抗菌和成骨诱导能力进一步提高，从而显著促进未感染和感染模型中的成骨。结论：我们确定NP层比NT结构具有更强的结合强度，相应的NP材料可能比NT更适合于制备用于骨损伤治疗的药物-装置组合钛植入物。
Background: Many studies have shown that the size of nanotube (NT) can significantly affect the behavior of osteoblasts on titanium-based materials. But the weak bonding strength between NT and substrate greatly limits their application. Purpose: The objective of this study was to compare the stability of NT and nanopore (NP) coatings, and further prepare antibacterial titanium-based materials by loading LL37 peptide in NP structures. Methods: The adhesion strength of NT and NP layers was investigated using a scratch tester. The proliferation and differentiation of MC3T3-E1 cells on different substrates were evaluated in vitro by CCK8, alkaline phosphatase activity, mineralization and polymerase chain reaction assays. The antibacterial rates of NP and NP/LL37 were also measured by spread plate method. Moreover, the osteogenesis around NP and NP/LL373 in vivo was further evaluated using uninfected and infected models. Results: Scratch test proved that the NP layers had stronger bonding strength with the substrates due to their continuous pore structures and thicker pipe walls than the independent NT structures. In vitro, cell results showed that MC3T3-E1 cells on NP substrates had better early adhesion, spreading and osteogenic differentiation than those of NT group. In addition, based on the drug reservoir characteristics of porous materials, the NP substrates were also used to load antibacterial LL37 peptide. After loading LL37, the antibacterial and osteogenic induction abilities of NP were further improved, thus significantly promoting osteogenesis in both uninfected and infected models. Conclusion: We determined that the NP layers had stronger bonding strength than NT structures, and the corresponding NP materials might be more suitable than NT for preparing drug-device combined titanium implants for bone injury treatment.