STRUCTURE AND FUNCTION OF RESTRICTION ENDONUCLEASES
STRUCTURE AND FUNCTION OF RESTRICTION ENDONUCLEASES
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DOI：
10.1016/0959-440x(95)80004-k
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发表时间：
1995-02-01
影响因子：
6.8
通讯作者：
AGGARWAL, AK
中科院分区：
文献类型：
--
作者：
AGGARWAL, AK
两种限制性内切酶BamHI和PvuII的结构在过去一年中被报道。这使目前已知的限制性内切酶结构的数量从2个增加到4个，并使它们的结构和DNA识别模式的比较分析成为可能。尽管这两种酶之间缺乏序列同源性，但BamHI与EcoRI相似，而PvuII与EcoRV相似。这四种酶的活性位点区域在结构上是相似的，但它们的裂解机制可能不同。
Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past year. This doubles the number of restriction endonuclease structures now known from two to four, and enables a comparative analysis of their structures and modes of DNA recognition. Despite a lack oi sequence homology between the enzymes, BamHI turns out to resemble EcoRI, and PvuII turns out to resemble EcoRV. The active-site regions are structurally similar in all four enzymes, but their mechanisms of cleavage may differ.