SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C-elegans
SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C-elegans
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DOI：
10.1016/s0092-8674(00)81227-1
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发表时间：
1998-07-10
期刊：
影响因子：
64.5
通讯作者：
Han, M
中科院分区：
文献类型：
--
作者：
Sieburth, DS;Sun, Q;Han, M
我们描述了一个新的基因，sur-8，正调控Ras介导的信号转导过程中的C。外阴发育。减少sur-8功能抑制激活的ras突变，并显着增强mpk-1 MAP激酶和ksr-1突变的表型，而增加sur-8剂量增强激活的ras突变。sur-8似乎在ras下游或平行作用，但在raf上游作用。sur-8编码主要由富含亮氨酸的重复序列组成的保守蛋白。SUR-8蛋白直接与Ras相互作用，但不与Ras（P34 G）突变蛋白相互作用，表明SURd可能通过Ras结合介导其作用。人体中的结构和功能SUR-8同源物在体外特异性结合K-Ras和N-Ras，但不结合H-Ras。
We describe the identification and characterization of a novel gene, sur-8, that positively regulates Ras-mediated signal transduction during C. elegans Vulval development. Reduction of sur-8 function suppresses an activated ras mutation and dramatically enhances phenotypes of mpk-1 MAP kinase and ksr-1 mutations, while increase of sur-8 dosage enhances an activated ras mutation. sur-8 appears to act downstream of or in parallel to ras but upstream of raf. sur-8 encodes a conserved protein that is composed predominantly of leucine-rich repeats. The SUR-8 protein interacts directly with Ras but not with the Ras(P34G) mutant protein, suggesting that SURd may mediate its effects through Ras binding. A structural and functional SUR-8 homolog in humans specifically binds K-Ras and N-Ras but not H-Ras in vitro.