Dynamics of soil protozoa using a direct count method
Dynamics of soil protozoa using a direct count method
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DOI：
10.1007/s00374-005-0009-x
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发表时间：
2005-11-01
影响因子：
6.5
通讯作者：
Coleman, DC
中科院分区：
文献类型：
--
作者：
Adl, SM;Coleman, DC
我们采用了一种直接计数的方法来获得活跃的原生动物的计数，而不是过于耗时。来自农业领域的土壤样本在一年中每隔1至3天检查三次。这三个采样期代表了不同的天气状况。在每次采样活动中，新鲜的土壤样本在返回实验室时被提取以检测原生动物。这些细菌在显微镜下计数，没有事先培养，在土壤-水悬浮液中稀释。我们描述了一种允许在几个小时内处理所有样品的程序。我们的结果表明，同一天采集的样品之间具有良好的重复性和一致性。我们的数据解决了几天之间的差异，因为土壤条件随着干燥或潮湿而变化缓慢。该方法适用于描述采样时的活性物种。与依赖于可培育物种的“最可能数量”程序不同，它不太容易计数过多的个体，而且它在采样日期之间提供了更好的分辨率，样本数相对较少。
We adapted a direct count method for obtaining counts of active protozoa that was not overly time consuming. Soil samples from an agricultural field were examined at 1- to 3-day intervals three times through the year. The three sampling periods represented different weather conditions. At each sampling event, fresh soil samples were extracted upon return to the laboratory for protozoa. These were enumerated at the microscope without prior culture, in soil-water suspension dilutions. We describe a procedure that allowed all samples to be processed in a few hours. Our results suggest there is good reproducibility and agreement between samples collected on the same day. Our data resolve differences between days as soil conditions changed slowly with drying or wetting. This procedure is suitable for describing species active at the time of sampling. Unlike the 'most probable number' procedure that relies on cultivable species, it is less prone to enumerating excysting individuals, and it provides better resolution between sampling dates, with a relatively low number of samples.