Regulation by nectin of the velocity of the formation of adherens junctions,and tight junctions
Regulation by nectin of the velocity of the formation of adherens junctions,and tight junctions
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DOI：
10.1016/s0006-291x(03)00919-7
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发表时间：
2003-06-20
影响因子：
3.1
通讯作者：
Takai, Y
中科院分区：
文献类型：
--
作者：
Honda, T;Shimizu, K;Takai, Y
钙粘蛋白是成纤维细胞和上皮细胞粘附连接 (AJ) 处关键的 Ca2+ 依赖性细胞间粘附分子，而密蛋白是上皮细胞紧密连接 (TJ) 处关键的 Ca2+ 独立细胞间粘附分子。 TJ的形成和维持依赖于AJ的形成和维持。 Nectin 是 Ca2+ 独立的免疫球蛋白样细胞粘附分子，由 nectin-1、-2、-3 和 -4 四个成员组成，参与与钙粘蛋白配合形成 AJ，以及随后形成 TJ。我们在此表明，在稳定表达 E-钙粘蛋白的 L 细胞和 Madin-Darby 犬肾细胞中，Nectin-1 的过度表达会增加基于 E-钙粘蛋白的 AJ 的形成速度，而通过在培养基中添加 nectin-1 抑制剂会降低基于 E-钙粘蛋白的 AJ 的形成速度。此外，在 Madin-Darby 犬肾细胞的培养基中，Nectin-1 的过度表达会增加基于 Claudin 的 TJ 的形成速度，而在培养基中添加 nectin-1 抑制剂则会降低基于 Claudin 的 TJ 的形成速度。这些结果表明，nectin 调节基于 E-钙粘蛋白的 AJ 的形成速度以及随后基于密蛋白的 TJ 的形成速度。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Cadherins are key Ca2+-dependent cell-cell adhesion molecules at adherens junctions (AJs) in fibroblasts and epithelial cells, whereas claudins are key Ca2+-independent cell-cell adhesion molecules at tight junctions (TJs) in epithelial cells. The formation and maintenance of TJs are dependent on the formation and maintenance of AJs. Nectins are Ca2+-independent immunoglobulin-like cell-cell adhesion molecules which comprise a family of four members, nectin-1, -2, -3, and -4, and are involved in the formation of AJs in cooperation with cadherins, and the subsequent formation of TJs. We show here that the velocity of the formation of the E-cadherin-based AJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in L cells stably expressing E-cadherin and Madin-Darby canine kidney cells. Moreover, the velocity of the formation of the claudin-based TJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in Madin-Darby canine kidney cells. These results indicate that nectins regulate the velocity of the formation of the E-cadherin-based AJs and the subsequent formation of the claudin-based TJs. (C) 2003 Elsevier Science (USA). All rights reserved.