Electrochemical catechol biosensor based on β-cyclodextrin capped gold nanoparticles and inhibition effect of ibuprofen
Electrochemical catechol biosensor based on β-cyclodextrin capped gold nanoparticles and inhibition effect of ibuprofen
复制标题
DOI：
10.1016/j.procbio.2021.06.004
复制
发表时间：
2021-06-12
影响因子：
4.4
通讯作者：
Toppare, Levent
中科院分区：
文献类型：
--
作者：
Kapan, Beste;Kurbanoglu, Sevinc;Toppare, Levent
在此基础上，提出并论证了一种基于酪氨酸酶(TYR)的纳米传感器表面修饰的β-环糊精修饰的金纳米颗粒。采用滴注法将β-环糊精修饰的金纳米粒子整合到石墨电极表面，将酪氨酸固定在修饰电极上，以邻苯二酚为底物作为药物抑制平台。对影响生物传感器响应的各种因素进行了优化研究，邻苯二酚的线性范围为1.56mM-25mM，检测下限为0.42mU M，灵敏度为2.094 mU M-1 cm(-2)，酪氨酸抑制后加入布洛芬药物活性化合物孵育15min，I-50值为213mM。该传感器具有简单的制造方法、优异的传感器性能和布洛芬抑制能力。结果表明，所设计的传感器是利用电化学方法检测Tyr抑制的理想标记物，布洛芬化妆品乳膏配方在化妆品市场上可能是一个可行的选择。
Herein, beta-cyclodextrin-capped gold nanoparticle surface designed for tymsinase (Tyr)-based nanosensor is proposed and demonstrated. Integration of the beta-cyclodextrin-capped gold nanoparticles on graphite electrode surface was achieved via drop-casting method, Tyr was immobilized on the modified electrode and then used as drug inhibition platform with catechol as the substrate. All optimization studies that affect biosensor response were conducted, and catechol was detected in the linear range of 1.56 mu M-25 mu M catechol concentration with a limit of detection of 0.42 mu M and sensitivity of 2.094 mu A.mu M-1.cm(-2).Tyr inhibition was followed with ibuprofen drug active compound with a 15 min incubation time, and the I-50 value was found as 213 mu M. The sensor is the first in the literature to use an electrochemical method for beta-cyclodextrin-capped gold nanoparticles based Tyr biosensor and ibuprofen inhibition. This sensor presents an easy fabrication method, excellent sensor properties, and ibuprofen inhibition capabilities. As a result, it is proposed that the designed sensor is an ideal marker for detecting Tyr inhibition using electrochemical methods and an ibuprofen-based cosmetic cream formulation could be a viable option in the cosmetic market.