Hi-D: nanoscale mapping of nuclear dynamics in single living cells
Hi-D: nanoscale mapping of nuclear dynamics in single living cells
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DOI：
10.1186/s13059-020-02002-6
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发表时间：
2020-04-20
期刊：
影响因子：
12.3
通讯作者：
Bystricky, Kerstin
中科院分区：
文献类型：
--
作者：
Shaban, Haitham A.;Barth, Roman;Bystricky, Kerstin
大量染色质运动尚未在高分辨率下分析。我们提出了Hi-D，一种方法来定量映射动态的染色质和丰富的核蛋白的每个像素同时在整个细胞核从荧光图像系列。Hi-D结合了染色质运动的重建和贝叶斯推理的局部扩散过程的分类。我们发现，在核内部的DNA动力学在空间上划分成0.3-3 μ m的结构域中的马赛克样的模式，从染色质压实解耦。这种模式因转录活性而重塑。Hi-D可以应用于任何致密和块状结构，为理解核分子的运动开辟了新的视角。
Bulk chromatin motion has not been analyzed at high resolution. We present Hi-D, a method to quantitatively map dynamics of chromatin and abundant nuclear proteins for every pixel simultaneously over the entire nucleus from fluorescence image series. Hi-D combines reconstruction of chromatin motion and classification of local diffusion processes by Bayesian inference. We show that DNA dynamics in the nuclear interior are spatially partitioned into 0.3-3-mu m domains in a mosaic-like pattern, uncoupled from chromatin compaction. This pattern was remodeled in response to transcriptional activity. Hi-D can be applied to any dense and bulk structures opening new perspectives towards understanding motion of nuclear molecules.