Willow genotype, but not drought treatment, affects foliar phenolic concentrations and leaf-beetle resistance
Willow genotype, but not drought treatment, affects foliar phenolic concentrations and leaf-beetle resistance
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DOI：
10.1111/j.0013-8703.2004.00199.x
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发表时间：
2004-10-01
影响因子：
1.9
通讯作者：
Weih, M
中科院分区：
文献类型：
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作者：
Glynn, C;Rönnberg-Wästljung, AC;Weih, M
在温室试验中，我们研究了杨柳基因型和灌溉制度（中度干旱和良好浇水）对植物生长参数，叶片氮和酚类浓度的影响，以及对蓝叶甲虫Phratora vulgatissima（L.）（鞘翅目：角叶甲科）。供试的10个无性繁殖杨柳基因型均为F2全同胞杂种，来源于柳柳（Salix viminalis（L.）（Salicaceae）（富含缩合单宁）和Salix dasyclados（L.）（杨柳科）（富含酚苷）。在培养皿中的离体叶上以及在完整盆栽植物上的自由生活的昆虫上进行昆虫生物测定。为期10周的灌溉处理导致盆栽杨柳树苗的表型差异具有统计学意义。总生物量略高于浇水处理。根总生物量的比例较高，在干旱处理的植物。叶片氮浓度存在显著的基因型差异，干旱处理植株叶片氮浓度较高。也有一个强大的基因型变异，在每一个酚类物质分析。缩合单宁，其中占总酚质量的最大比例，在良好的浇水处理较高。然而，灌溉处理之间的其他酚类化合物（水杨酸，肉桂酸，黄酮类化合物和绿原酸）的水平没有差异。这些酚类化合物的总和较高，在良好的浇水处理。不同基因型杨柳上的毛白杨幼虫发育存在很大差异，且幼虫生长表现与水杨酸和肉桂酸水平呈负相关。然而，灌溉处理对幼虫的性能没有影响。Phratora vulgatissima更喜欢在浇水充足的植物上取食，我们发现了产卵的偏好，但无论是取食还是产卵地点的偏好都不受杨柳基因型的影响。成虫的取食和产卵偏好与幼虫的表现无关。
In a greenhouse experiment we examined the effect of willow genotype and irrigation regime (moderate drought and well-watered) on plant growth parameters, foliar nitrogen, and phenolic concentrations, as well as on the preference and performance of the blue leaf beetle, Phratora vulgatissima (L.) (Coleoptera: Chrysomelidae). The 10 vegetatively propagated willow genotypes in the experiments were F2 full-sibling hybrids, originated from a cross between Salix viminalis (L.) (Salicaceae) (high in condensed tannins) and Salix dasyclados (L.) (Salicaceae) (rich in phenolic glycosides). Insect bioassays were conducted on detached leaves in Petri dishes as well as with free-living insects on intact potted plants. The 10-week long irrigation treatments caused statistically significant phenotypic differences in the potted willow saplings. Total biomass was somewhat higher in the well-watered treatment. The root to total biomass ratio was higher in the drought-treatment plants. There was significant genotypic variation in foliar nitrogen concentrations, and they were higher in the drought-treatment plants. There was also a strong genotypic variation in each of the phenolic substances analyzed. Condensed tannins, which accounted for the greatest proportion of total phenolic mass, were higher in the well-watered treatment. There was, however, no difference in levels of the other phenolics (salicylates, cinnamic acid, flavonoids, and chlorogenic acid) between irrigation treatments. The sum of these phenolics was higher in the well-watered treatment. There was a strong variation in P. vulgatissima larval development on different willow genotypes, and larval performance was negatively correlated with levels of salicylates and cinnamic acid. There was, however, no effect of irrigation treatment on larval performance. Phratora vulgatissima preferred to feed on well-watered plants, and we found a preference for oviposition there, but neither feeding nor oviposition site preference was affected by willow genotype. Adult feeding and oviposition preferences were not correlated with larval performance.