A near-infrared-emission aza-BODIPY-based fluorescent probe for fast, selective, and "turn-on" detection of HClO/ClO-
A near-infrared-emission aza-BODIPY-based fluorescent probe for fast, selective, and "turn-on" detection of HClO/ClO-
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DOI：
10.1016/j.talanta.2021.122581
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发表时间：
2021-06-05
期刊：
影响因子：
6.1
通讯作者：
Gao, Liqian
中科院分区：
文献类型：
--
作者：
Shi, Wen-Jing;Feng, Liu-Xia;Gao, Liqian
合成了一种新型的氮杂BODIPY荧光探针，并研究了其对次氯酸/次氯酸盐（HClO/ClO-）的荧光传感性能.由于两个碲原子的光诱导电子转移，该探针的荧光被充分猝灭。当暴露于HClO/ClO-时，在738 nm处出现强的近红外发射，荧光量子产率从0变化到0.11。这种显著的荧光变化归因于两个富电子碲原子的氧化。在0 - 30 μ M的HClO/ClO-浓度范围内，通过在738 nm处的线性荧光变化计算该探针对HClO/ClO-的检测限为0.09 μ M。有趣的是，发现该探针适用于宽pH范围（2 - 10）。同时，氧化的探针可以进一步响应于生物硫醇，具有显著的荧光消失。在RAW264.7细胞中的生物成像实验表明，加入HClO/ClO-和PMA后，细胞内出现了近红外荧光，进一步引入GSH后，荧光也可逆转至沉默，证实了其在活细胞中外源性和内源性HClO/ClO-检测的潜在应用价值。
A novel near-infrared-emitting aza-BODIPY-based fluorescent probe with two tellurium atoms at two upper benzyl rings has been prepared and explored for its fluorescent sensing properties towards hypochlomus acid/ hypochorite (HClO/ClO-), which showed high selectivity and absolutely fluorescent "turn-on" phenomenon at 738 nm. The fluorescence of this probe was sufficiently quenched due to photoindued electron transfer by two tellurium atoms. Upon exposure to HClO/ClO-, a strong near-infrared emission at 738 nm appeared with fluorescence quantum yields changing from 0 to 0.11. This remarkable fluorescence change was ascribed to the oxidation of both electron-rich tellurium atoms. The detection limit of this probe towards HClO/ClO- was calculated to 0.09 mu M in acetonitrile aqueous solution by the linear fluorescence change at 738 nm in the HClO/ClO--concentration range of 0-30 mu M. Interestingly, this probe was found to be applicable in a broad pH range (2-10). Meanwhile, the oxidized probe could be further responsive to biothiols with substantial fluorescence disappearance. The bioimaging experiments in RAW264.7 cells showed the appearance of intracellular nearinfrared fluorescence after addition of HClO/ClO- and PMA, and the fluorescence could also be reversed to be silenced by further introduction of GSH, confirming its potential application for exogenous and endogenous detection of HClO/ClO- in living cells.