Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation
Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation
复制标题
圆红冬孢酵母脂质积累过程中的比较蛋白质组学分析
DOI：
10.1002/yea.1706
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发表时间：
2009-10-01
期刊：
影响因子：
2.6
通讯作者：
Zou, Hanfa
中科院分区：
文献类型：
--
作者：
Liu, Hongwei;Zhao, Xin;Zou, Hanfa
细胞内脂质积累是一些真核微生物在特定生长条件下的常见生物学过程，但在“组学”水平上对这一现象的研究仍然很少。在这项研究中，我们通过将细胞转移到氮限制培养基中，诱导产油酵母toruloides的脂质积累，并通过2D-LC-MS/MS方法对此后获得的细胞样品进行了比较和半定量蛋白质组学分析。基于酵母数据库，共鉴定出184个蛋白。半定量分析表明，46种蛋白在脂质生成过程中发生了显著变化。其中有7个蛋白仅在晚期显著上调，3个蛋白在早期显著上调，4个蛋白在两期均显著上调。在两个阶段均有26种蛋白显著下调。下调蛋白主要与蛋白质代谢和碳水化合物代谢有关，上调蛋白主要与替代氮源代谢和脂质生物合成有关。我们的数据表明，缺氮环境对细胞代谢有关键影响，可能刺激了圆叶蝉脂质积累过程。这项工作为进一步探索细胞脂质代谢的分子机制提供了有价值的信息，并应引起产油微生物工程的极大兴趣。版权所有John Wiley & Sons， Ltd. 2009
Intracellular lipid accumulation is a common biological process for some eukaryotic microorganisms under specific growth conditions, yet study on this phenomenon at an '-omics' level remains rare. In this study we induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides by transferring cells into a nitrogen-limited medium and performed a comparative and semi-quantitative proteomic analysis of cell samples obtained thereafter by a 2D-LC-MS/MS approach. A total of 184 proteins were identified, based on the database of yeast Saccharomyces cerevisiae. Semi-quantitative analysis suggested that 46 proteins were notably changed during the lipid production process. Among them, seven, three and four proteins were significantly upregulated only at the late stage, the early stage and both stages, respectively. There were 26 proteins drastically downregulated at both stages. The majority of the downregulated proteins are related to protein metabolism and carbohydrate metabolism, whereas the upregulated proteins are mainly involved in alternative nitrogen sources metabolism and lipid biosynthesis. Our data indicated that a nitrogen deficiency environment had a key impact on cellular metabolism that likely stimulated the lipid accumulation process by R. toruloides. This work provids valuable information for further exploration of the molecular mechanism of cellular lipid metabolism and should be of great interest in oleaginous microorganisms engineering. Copyright (C) 2009 John Wiley & Sons, Ltd.