Chromatographic separation and photometric analysis of the components of Nile blue sulphate.
Chromatographic separation and photometric analysis of the components of Nile blue sulphate.
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尼罗蓝硫酸盐组分的色谱分离和光度分析。
DOI：
10.3109/10520296809115076
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
M. Dunnigan
中科院分区：
文献类型：
--
作者：
M. Dunnigan
以硫酸耐尔蓝（Geigy和G. T. Gurr）进行薄层层析分离。发现蓝色硫酸化恶嗪碱由在可见光范围内具有相同吸收光谱的3种组分组成。这些可能代表染料的同分异构形式。红色恶嗪酮作为单一组分传播。至少有6个次要成分，存在少量，被确定。分光光度法测定结果表明，硫酸化恶嗪碱有3个最大吸收峰。吸收光谱受pH、染料浓度、染料溶剂和物理状态的影响。还测量了恶嗪酮和游离恶嗪碱的吸收光谱。这些显示出明显的溶剂化变色效应，随着溶剂极性的降低，吸收最大值移动到较短的波长。
Samples of Nile blue sulphate (Geigy and G. T. Gurr) were separated by silicic acid thin layer chromatography. The blue sulphated oxazine base was found to consist of 3 components with identical absorption spectra in the visible range. These probably represent isomeric forms of the dye. The red oxazone travelled as a single component. At least 6 minor constituents, present in small amount, were identified. Spectrophotometric examination showed that the sulphated oxazine base has 3 absorption maxima. The absorption spectrum is influenced by pH, dye concentration, dye solvent, and physical state. The absorption spectra of the oxazone and free oxazine base were also measured. These showed marked solvatochromic effects, absorption maxima moving to shorter wavelengths with decreasing solvent polarity.