Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels.
Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels.
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DOI：
10.1006/abio.1999.4326
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发表时间：
1999-11
影响因子：
2.9
通讯作者：
Robley C. Williams;Chirayu Shah;D. Sackett
中科院分区：
文献类型：
--
作者：
Robley C. Williams;Chirayu Shah;D. Sackett
方法：研究方法。在由7M尿素、2M硫脲、20 mM DTT、4%CHAPS、0.5%Triton X-100和0.5%IPG缓冲液组成的运行溶液(14)中，利用IPGphor装置及其相关条架(amersham Pharmacia Biotech，Piscataway，NJ)，在预制的固定化干凝胶条带(pH 4~7，长11 cm)中进行等电聚焦(IEF)2
Methods. Isoelectric focusing (IEF) 2 was carried out in precast Immobiline DryStrip gel strips (pH 4–7, 11 cm long) by means of an IPGphor apparatus and its associated strip holders (Amersham Pharmacia Biotech, Piscataway, NJ) in a running solution (14) composed of 7 M urea, 2 M thiourea, 20 mM DTT, 4% Chaps, 0.5% Triton X-100, and 0.5% IPG buffer, pH