INHIBITION OF COAGGREGATION BETWEEN FUSOBACTERIUM-NUCLEATUM AND PORPHYROMONAS (BACTEROIDES) GINGIVALIS BY LACTOSE AND RELATED SUGARS
INHIBITION OF COAGGREGATION BETWEEN FUSOBACTERIUM-NUCLEATUM AND PORPHYROMONAS (BACTEROIDES) GINGIVALIS BY LACTOSE AND RELATED SUGARS
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DOI：
10.1128/iai.57.10.3204-3209.1989
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发表时间：
1989-10-01
影响因子：
3.1
通讯作者：
ANDERSEN, RN
中科院分区：
文献类型：
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作者：
KOLENBRANDER, PE;ANDERSEN, RN
乳糖、n -乙酰- d -半乳糖胺和d -半乳糖均能很好地抑制牙龈卟啉单胞菌PK1594和牙龈卟啉单胞菌PK1924的共聚集，在2 mM糖浓度下，共聚集的抑制率达到50%。其他糖如D-半乳糖胺、D-焦糖（6-脱氧-D-半乳糖）和α。-甲基和。-甲基- d -半乳糖苷也抑制共聚集。糖的特异性很明显，因为L-病灶、L-鼠李糖、n -乙酰- d -葡萄糖胺和n -乙酰神经氨酸都不是抑制剂。蛋白酶处理梭杆菌完全消除了共聚集，而对卟啉单胞菌的共聚集活性没有影响。虽然已知革兰氏阳性细菌中存在许多乳糖抑制的聚集对，但本报告和随附的调查（P. E. Kolenbrander， R. N. Andersen和L. V. H. Moore，《感染》）。免疫（57:3194-3203,1989）是第一个证明革兰氏阴性人类口腔细菌之间这种相互作用的广泛性质的研究。讨论了半乳糖苷抑制这两种潜在牙周病原体聚集的意义。
The coaggregation of Fusobacterium nucleatum PK1594 and Porphyromonas (Bacteriodes) gingivalis PK1924 was inhibited equally well by lactose, N-acetyl-D-galactosamine, and D-galactose, which caused 50% inhibition of coaggregation at 2 mM sugar concentration. Other sugars such as D-galactosamine, D-fucose (6-deoxy-D-galactose), and .alpha.-methyl- and .beta.-methyl-D-galactosides also inhibited coaggregation. Sugar specificity was apparent, since neither L-fucose, L-rhamnose, N-acetyl-D-glucosamine, nor N-acetylneuraminic acid was an inhibitor. Protease treatment of the fusobacterium completely abolished coaggregation, whereas it had no effect on the coaggregating activity of the porphyromonad. Although numerous lactose-inhibitable coaggregating pairs are known to occur among gram-positive bacteria, this report and the accompanying survey (P. E. Kolenbrander, R. N. Andersen, and L. V. H. Moore, Infect. Immun. 57:3194-3203, 1989) are the first studies demonstrating the extensive nature of this type of interaction between gram-negative human oral bacteria. The significance of galactoside-inhibitable coaggregations between these two potential periodontal pathogens is discussed.