SPECIFICITY OF A MONOCLONAL-ANTIBODY FOR THE NH2-TERMINAL REGION OF FIBRIN
SPECIFICITY OF A MONOCLONAL-ANTIBODY FOR THE NH2-TERMINAL REGION OF FIBRIN
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DOI：
10.1016/0161-5890(84)90093-2
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
WIEBE, ME
中科院分区：
文献类型：
--
作者：
KUDRYK, B;ROHOZA, A;WIEBE, ME
使用来自用人纤维蛋白II的NH 2-末端CNBr片段，即所谓的（T）N-DSK [（A α 17 -51，B β 15 -118，γ 1 -78）2]免疫的小鼠的脾细胞，通过融合制备单克隆抗体（Mab/T2 G1 s）。在竞争实验中，该抗体与（T）N-DSK以及肽B β 15 -42反应，肽B β 15 -42可以通过用血浆消化从（T）N-DSK获得。与完整的纤维蛋白原、分别来自纤维蛋白原（N-DSK）或纤维蛋白I[（B）N-DSK]的NH 2-末端CNBr片段以及肽B β观察到很少或没有反应。1-42.显然，MAb/T2 G针对B β上的表位。链在纤维蛋白II中，但不在纤维蛋白原或纤维蛋白I中。因此，MAb/T2 G1与另一种抗体（MAb/1- 8 C6）完全不同，所述另一种抗体也对B β的NH 2-末端区域具有特异性。链，最近由Kudryk等人描述。
A monoclonal antibody (Mab/T2G1s) was prepared by fusion using spleen cells from mice immunized with the NH2-terminal CNBr fragment of human fibrin II, the so-called (T)N-DSK [(A.alpha.17-51, B.beta.15-118, .gamma.1-78)2]. In competition experiments, this antibody reacted with (T)N-DSK as well as peptide B.beta.15-42 which can be obtained from (T)N-DSK by digestion with plasma. Little or no reaction was observed with intact fibrinogen, the NH2-terminal CNBr fragments from fibrinogen (N-DSK) or fibrin I[(B)N-DSK], respectively, as well as peptide B.beta. 1-42. Evidently, MAb/T2G is directed to an epitope on the B.beta. chain in fibrin II but not in fibrinogen or fibrin I. As such, MAb/T2G1s differs completely from another antibody (MAb/1-8C6), also specific for the NH2-terminal region of the B.beta. chain which was recently described by Kudryk et al.