Evaluation of T-lymphocyte subsets present in semen and peripheral blood of healthy donors: a report from the heterosexual transmission study.
Evaluation of T-lymphocyte subsets present in semen and peripheral blood of healthy donors: a report from the heterosexual transmission study.
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健康捐献者精液和外周血中 T 淋巴细胞亚群的评估：异性传播研究的报告。
DOI：
10.1002/cyto.990200411
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Colon,J
中科院分区：
文献类型：
--
作者：
Denny,TN;Scolpino,A;Garcia,A;Polyak,A;Weiss,SN;Skurnick,JH;Passannante,MR;Colon,J
本研究的目的是准确测定健康志愿者精液中的T淋巴细胞亚群，评估重复射精对精液中免疫细胞频率或类型的影响，并比较精液和外周血中的亚群分析。为了实现这一点，开发了流式细胞术方法来识别和计数精液中存在的免疫表型不同的细胞。连续三天从9名健康供体中采集新鲜精液样本和外周血。供者的射精量、精子计数、精子活力、形态和白细胞计数均正常。随着时间的推移，这些参数未观察到显著的供体内差异。精液中CD 3+细胞、CD 4+细胞、CD 8+细胞百分比及CD 4：CD 8比值在连续几天内均无显著性差异。然而，在CD 4+亚群中，当测量初始和记忆CD 4+细胞时，提示存在一些日间变异性。在采样日之间，外周血中未观察到CD 3+、CD 4+、CD 8+、CD 4/CD 8比值或幼稚和记忆亚群的显著差异。当精液与外周血相比时，观察到免疫亚群值的一些差异，其中精液中记忆CD 4+细胞百分比的增加最为显著。这一发现可能与HIV传播有关，因为其他人已经表明，这种细胞可能优先感染HIV，并且是感染个体中病毒的主要储存库。© 1995 Wiley利斯公司
The purpose of this study was to accurately determine the T‐lymphocyte subsets found in semen from health volunteers, to evaluate the impact of repeated ejaculation on the frequency or type of immune cells present in semen, and to compare subset analysis in semen to that in the peripheral blood. To accomplish this, a flow cytometric method was developed to identify and count immunophenotypically distinct cells present in semen. Fresh semen samples and peripheral blood were collected over three consecutive days from nine healthy donors. Donors had normal ejaculate volume, sperm count, sperm motility, morphology, and leukocyte count. No significant intra‐donor differences were seen in these parameters over time. No significant differences were observed in the percentage of CD3+cells, CD4+cells, CD8+cells, and the CD4:CD8 ratio in semen on consecutive days. However, within the CD4+subset, when naive and memory CD4+cells were measured, some day to day variability was suggested. No significant differences in CD3+, CD4+, CD8+, CD4/CD8 ratio, or naive and memory subsets were seen in the peripheral blood between sampling days. When semen was compared to peripheral blood some differences in immune subset values were observed, with an increase in the percentage of memory CD4+cells in semen being the most striking. This finding may be relevant to HIV transmission, since others have shown that this cell may be preferentially infected with HIV and is the primary reservoir for virus in infected individuals. © 1995 Wiley‐Liss, Inc.