Volumetric stability of lipid bilayers
Volumetric stability of lipid bilayers
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DOI：
10.1039/c2cp42595e
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Nagle, John F.
中科院分区：
文献类型：
--
作者：
Hallinen, Kelsey M.;Tristram-Nagle, Stephanie;Nagle, John F.
与最近的报道一致，商业密度计已经在10 ℃和50 ℃之间的重复热循环时产生了凝胶相中DPPC多层囊泡分散体的脂质分子体积的逐渐减小。这种减少的相当大的规模将对生物膜的物理化学产生重大影响。相比之下，用类似的热循环进行的中性浮力测量表明在20 ℃下凝胶相中的脂质体积没有逐渐变化。在密度计中重新混合脂质表明，表观体积减少是人为因素。我们得出结论，凝胶相DPPC双层存在于体积稳定相。
In agreement with recent reports, a commercial densimeter has yielded a gradual decrease in lipid molecular volume of DPPC multilamellar vesicle dispersions in the gel phase upon repeated thermal cycling between 10 degrees C and 50 degrees C. The considerable size of this decrease would have significant implications for the physical chemistry of biomembranes. In contrast, neutral buoyancy measurements performed with similar thermal cycling indicate no gradual change in lipid volume in the gel phase at 20 degrees C. Remixing the lipid in the densimeter shows that the apparent volume decrease is an artifact. We conclude that gel phase DPPC bilayers exist in a volumetrically stable phase.