Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications
Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications
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DOI：
10.1088/0957-4484/23/9/095705
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发表时间：
2012-03-09
期刊：
影响因子：
3.5
通讯作者：
Ramakrishna, Seeram
中科院分区：
文献类型：
--
作者：
Kai, Dan;Prabhakaran, Molamma P.;Ramakrishna, Seeram
基于水凝胶的生物材料系统作为组织工程（TE）的临时支架和细胞递送载体，在组织重建方面具有巨大的潜力。水凝胶力学性能差，降解快，限制了水凝胶在TE中的发展和应用。在这项研究中，通过将静电纺丝聚（ε-己内酯）（PCL）/明胶的“共混物”或“同轴”纳米纤维到明胶水凝胶中，制备了聚己内酯增强复合水凝胶。结果表明，纳米复合水凝胶的模量和压缩强度均明显高于纯明胶水凝胶。通过增加掺入到水凝胶中的纳米纤维的量，复合水凝胶的杨氏模量从3.29 +/-1.02kPa增加到20.30 +/-1.79kPa，而断裂应变从66.0 +/- 1.1%降低到52.0 +/-3.0%。与同轴纳米纤维复合水凝胶相比，共混纳米纤维复合水凝胶具有更高的压缩强度和断裂应变，但模量和能量耗散性能较低。采用骨髓间充质干细胞（BM-MSCs），通过细胞增殖试验和免疫染色分析，对生物相容性进行评价。发现含有25 mg/ml PCL/明胶“共混物”纳米纤维（PGB 25）的纳米复合水凝胶可增强细胞增殖，表明“纳米复合水凝胶”可提供必要的机械支撑，并可能是用于组织再生的有前景的细胞递送系统。
Hydrogel-based biomaterial systems have great potential for tissue reconstruction by serving as temporary scaffolds and cell delivery vehicles for tissue engineering (TE). Hydrogels have poor mechanical properties and their rapid degradation limits the development and application of hydrogels in TE. In this study, nanofiber reinforced composite hydrogels were fabricated by incorporating electrospun poly(epsilon-caprolactone) (PCL)/gelatin 'blend' or 'coaxial' nanofibers into gelatin hydrogels. The morphological, mechanical, swelling and biodegradation properties of the nanocomposite hydrogels were evaluated and the results indicated that the moduli and compressive strengths of the nanofiber reinforced hydrogels were remarkably higher than those of pure gelatin hydrogels. By increasing the amount of incorporated nanofibers into the hydrogel, the Young's modulus of the composite hydrogels increased from 3.29 +/- 1.02 kPa to 20.30 +/- 1.79 kPa, while the strain at break decreased from 66.0 +/- 1.1% to 52.0 +/- 3.0%. Compared to composite hydrogels with coaxial nanofibers, those with blend nanofibers showed higher compressive strength and strain at break, but with lower modulus and energy dissipation properties. Biocompatibility evaluations of the nanofiber reinforced hydrogels were carried out using bone marrow mesenchymal stem cells (BM-MSCs) by cell proliferation assay and immunostaining analysis. The nanocomposite hydrogel with 25 mg ml(-1) PCL/gelatin 'blend' nanofibers (PGB25) was found to enhance cell proliferation, indicating that the 'nanocomposite hydrogels' might provide the necessary mechanical support and could be promising cell delivery systems for tissue regeneration.