A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl
A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl
复制标题
正常色素白来亨鸡和色素过度突变的乌骨鸡黑素细胞迁移过程中基因表达改变的微阵列研究
DOI：
10.15302/j-fase-2014040
复制
发表时间：
2014-12-01
影响因子：
3.7
通讯作者：
Deng, Xuemei
中科院分区：
文献类型：
--
作者：
Li, Yulin;Han, Deping;Deng, Xuemei
源自神经嵴细胞的黑素母细胞可以通过发育胚胎的间质迁移并产生黑素细胞。与其他脊椎动物的黑素细胞局限于被膜不同，由于成黑素细胞迁移过程中基因表达的差异，丝鸡的黑素细胞可以到达胚胎的腹侧区域。在这项研究中，我们使用微阵列分析来鉴定白来窝鸡和乌鸡之间基因表达的差异。2517微阵列探针（P2）在乌鸡和白来窝鸡中的表达差异。通过聚类分析、功能注释和通路分析筛选出8个与黑素细胞发育密切相关的差异表达基因。此外，还发现了乌鸡和白来窝鸡免疫基因的表达差异。通过q-PCR验证了与黑素细胞发育相关的差异表达基因，结果与微阵列数据高度一致。这些与黑素细胞迁移和发育相关的基因表达显著改变表明，尽管两个品种的黑素细胞发育差异不大，但不同的微环境导致了黑素细胞迁移异常。本研究发现的候选基因有助于了解丝鸡色素沉着的分子机制。
Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes. Unlike the melanocytes that are confined to the integument in other vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration. In this study, we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl. Differential expression of 2517 microarray probes (P2) was observed in Silky Fowl compared to White Leghorn. After filtration by cluster analysis, functional annotation and pathway analysis, eight differentially expressed genes were identified to be closely related to the development of melanocytes. Moreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn. The differentially expressed genes associated with melanocyte development were verified by q-PCR, and results were highly consistent with the microarray data. The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl, although there were no big differences in melanoblast development between these two breeds. The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.