COMBINED ACTION OF STEM-CELL FACTOR, LEUKEMIA INHIBITORY FACTOR, AND CAMP ON INVITRO PROLIFERATION OF MOUSE PRIMORDIAL GERM-CELLS
COMBINED ACTION OF STEM-CELL FACTOR, LEUKEMIA INHIBITORY FACTOR, AND CAMP ON INVITRO PROLIFERATION OF MOUSE PRIMORDIAL GERM-CELLS
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DOI：
10.1002/mrd.1080350206
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发表时间：
1993-06-01
影响因子：
2.5
通讯作者：
DEFELICI, M
中科院分区：
文献类型：
--
作者：
DOLCI, S;PESCE, M;DEFELICI, M
本文研究了干细胞因子/肥大细胞生长因子（SCF/MGF）对体外培养的人乳腺癌细胞的作用，白血病抑制因子/分化抑制活性（已知影响体外原始生殖细胞生长的两种生长因子）和毛喉素（FRSK）（在许多细胞类型中的腺苷酸环化酶的激活剂）单独或组合对从8.5获得的原始生殖细胞（PGCs）的存活和增殖的影响，结果表明，在培养的第1天和第3天，加入100 ng/ml SCF，20 μ M FRSK，或在某些情况下单独加入20 ng/ml LIF，与对照组相比，均引起PGC数量的显著增加。当SCF和/或LIF与FRSK一起使用时获得最高效果。此外，我们还发现FRSK可提高纯化的11.5dpc PGCs中cAMP的水平，而SCF和LIF却不能促进PGC的增殖，这一结果提示cAMP与SCF和/或LIF联合作用于体外控制小鼠PGCs增殖的机制。
In the present paper we investigated the effects of stem cell factor/mastocyte growth factor (SCF/MGF), leukemia inhibitory factor/differentiating inhibitory activity (LIF/DIA) (two growth factors known to affect primordial germ cell growth in vitro) and forskolin (FRSK) (an activator of-adenylate cyclase in many cell types) alone or in combination on the survival and proliferation of primordial germ cells (PGCs) obtained from 8.5, 10.5, and 11.5 days post coitum (dpc) mouse embryos and cultured without pre-formed cell feeder layers.The results showed that both at 1 and 3 days of culture the addition of 100 ng/ml SCF, 20 muM FRSK, or in some instances 20 ng/ml LIF alone caused a significant increase of PGC number as compared with controls. The highest effects were obtained when SCF and/or LIF were used together with FRSK. Moreover, we found that FRSK elevated cAMP levels in purified 11.5 dpc PGCs and that this compound, but not SCF and LIF, stimulated PGC proliferation, as assessed by 5-bromo-2'-deoxyuridin (BrdU) incorporation.These results suggest a mechanism of combined action of cAMP with SCF and/or LIF in the control of proliferation of mouse PGCs in vitro.