Role of glutamate receptors in the ventromedial hypothalamus in the regulation of female rat sexual behaviors - I. Behavioral effects of glutamate and its selective receptor agonists AMPA, NMDA and kainate
Role of glutamate receptors in the ventromedial hypothalamus in the regulation of female rat sexual behaviors - I. Behavioral effects of glutamate and its selective receptor agonists AMPA, NMDA and kainate
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DOI：
10.1016/j.pbb.2006.02.016
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发表时间：
2006-02-01
影响因子：
3.6
通讯作者：
Pfaus, JG
中科院分区：
文献类型：
--
作者：
Georgescu, M;Pfaus, JG
双侧下丘脑腹内侧部(VMH)注入谷氨酸或海人酸能迅速抑制雌激素刺激的雌性大鼠手部刺激所致的前凸。本研究检测了谷氨酸或选择性离子营养谷氨酸受体激动剂是否能破坏雌性大鼠在与雄性交配时双侧VMH输注后的食欲和完成性行为。去势性经验丰富的雌性大鼠双侧植入指向腹外侧侧VMH的导向管。术后恢复后，雌鼠用苯甲酸雌二醇和孕酮预注，并在与性欲旺盛的雄鼠在双层室内进行测试前3min分别注入不同剂量的谷氨酸、AMPA、NMDA或海人藻酸(n=9~10)。注入谷氨酸减少了啤酒花和飞镖的显示，并产生了减少前凸的趋势。注入AMPA减少了引诱、跳跃和飞镖以及前凸的显示。NMDA输注减少了前凸，增加了防御行为和起搏。海人藻酸盐的注入减少了引诱、跳跃和飞镖，以及前凸，增加了防御行为和步调。这些数据表明，VMH中谷氨酸受体的激活对雌性大鼠性行为的食欲和性欲方面都是抑制的。(C)2006 Elsevier Inc.保留所有权利。
Bilateral infusions of glutamate or kainate to the ventromedial hypothalamus (VMH) have been shown previously to produce a rapid inhibition of lordosis in estrogen-primed female rats induced by manual flank stimulation. The present study examined whether glutamate or selective ionotrophic glutamate receptor agonists can disrupt appetitive and consummatory sexual behaviors following bilateral infusions to the VMH of females during copulation with male rats. Ovariectomized, sexually experienced female rats were implanted bilaterally with guide cannulae aimed at the ventrolateral VMH. After recovery from surgery, females were primed with estradiol benzoate and progesterone and infused with different doses of glutamate, AMPA, NMDA or kainate (n=9-10 in each dose group) 3 min prior to a test with sexually vigorous males in bilevel chambers. Glutamate infusions decreased the display of hops and darts and produced a trend toward decreased lordosis. AMPA infusions decreased the display of solicitations, hops and darts, and lordosis. NMDA infusions decreased lordosis and increased defensive behaviors and pacing. Kainate infusions decreased solicitations, hops and darts, and lordosis, and increased defensive behaviors and pacing. These data indicate that the activation of glutamate receptors in the VMH is inhibitory for both appetitive and consummatory aspects of sexual behavior in the female rat. (c) 2006 Elsevier Inc. All rights reserved.