Temporal and spatial profiles of ABCA2‐expressing oligodendrocytes in the developing rat brain
Temporal and spatial profiles of ABCA2‐expressing oligodendrocytes in the developing rat brain
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发育中的大鼠大脑中表达 ABCA2 的少突胶质细胞的时间和空间分布
DOI：
10.1002/cne.10493
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发表时间：
2003
影响因子：
2.5
通讯作者：
N. Inagaki
中科院分区：
文献类型：
--
作者：
Yukiko Tanaka;Katsuya Yamada;Cheng‐Ji Zhou;N. Ban;S. Shioda;N. Inagaki
ABCA2蛋白属于ATP结合盒（ABC）转运蛋白的ABCA亚类，被认为在内源性脂质的跨膜转运中发挥关键作用。在这项研究中，我们通过免疫印迹分析发现，大约260 kDa的ABCA2蛋白主要在少突胶质细胞中表达，并且在成熟过程中，特别是在出生后6天至19天之间，该蛋白在大脑中的表达上调。与ABCA2表达的变化平行，免疫组织化学分析显示，在此期间，ABCA2免疫标记的少突胶质细胞在大脑中快速的空间扩散。这些ABCA2表达的时间和空间变化与先前报道的骨髓结构学结果很好地一致。此外，ABCA2和髓鞘的主要结构蛋白髓鞘碱性蛋白的双重免疫标记表明，ABCA2在少突胶质细胞中的表达与髓鞘碱性蛋白免疫标记的厚髓鞘段的出现一致。由于ABCA2在成人皮层白质和灰质中大量表达，因此研究了ABCA2与少突胶质祖细胞NG2或血小板衍生生长因子α受体的共同表达。没有观察到共表达ABCA2和标记物的细胞，这表明ABCA2主要表达在髓磷脂形成的少突胶质细胞中，与测试的成人少突胶质祖细胞不同。这些结果表明ABCA2在底物（如与髓鞘形成过程密切相关的脂质）的膜运输中起作用。中国生物医学工程学报，2004,25(5):357 - 367。©2002 Wiley‐Liss, Inc。
ABCA2 protein belongs to the ABCA subclass of ATP‐binding cassette (ABC) transporters proposed to exert critical functions in transmembrane transport of endogenous lipids. In this study, we found by immunoblot analyses that approximately 260 kDa of ABCA2 protein is expressed predominantly in oligodendrocytes, and that the expression of the protein is upregulated in the brain during maturation, especially between postnatal days 6 and 19. Parallel to the changes in expression of ABCA2, immunohistochemical analyses showed rapid spatial spread of ABCA2‐immunolabeled oligodendrocytes in the brain during this period. These temporal and spatial changes in ABCA2 expression were in good agreement with findings in myeloarchitectonics reported previously. Further, double immunolabeling with ABCA2 and a major structural protein of myelin, myelin basic protein, demonstrated that onset of ABCA2 expression in oligodendrocytes coincides with the appearance of thick myelin segments immunolabeled with myelin basic protein. Because ABCA2 was abundantly expressed in adult cortex in white matter and gray matter, coexpression of ABCA2 and a marker for the oligodendroglial progenitors NG2 or platelet‐derived growth factor α receptor was investigated. No cells coexpressing ABCA2 and the marker were observed, suggesting that ABCA2 is expressed predominantly in myelin‐forming oligodendrocytes distinct from the adult oligodendroglial progenitors tested. These results suggested a role for ABCA2 in membrane transport of substrates such as the lipids that are closely linked to myelination processes. J. Comp. Neurol. 455:353–367, 2003. © 2002 Wiley‐Liss, Inc.
DOI：
10.1152/ajplung.1992.263.4.l479
发表时间：
1992-10
期刊：
The American journal of physiology
影响因子：
--
作者：
W. Voorhout;T. Veenendaal;H. Haagsman;T. Weaver;J. Whitsett;L.M.G. Van Golde;H. Geuze
通讯作者：
W. Voorhout;T. Veenendaal;H. Haagsman;T. Weaver;J. Whitsett;L.M.G. Van Golde;H. Geuze
人腺苷 5-三磷酸结合盒、亚家族 A、转运蛋白 2 (ABCA2) 的克隆和表征。
DOI：
--
发表时间：
2001
期刊：
Cancer research.
影响因子：
--
作者：
Vulevic,B;Chen,Z;Boyd,JT;DavisJr,W;Walsh,ES;Belinsky,MG;Tew,KD
通讯作者：
Tew,KD