Perchlorate Reduction by Autotrophic Bacteria in the Presence of Zero-Valent Iron
Perchlorate Reduction by Autotrophic Bacteria in the Presence of Zero-Valent Iron
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DOI：
10.1021/es051682z.s001
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发表时间：
2006
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X. U E Y U A N Y U, † C H R I S T O P H E R A M R H E-X.-U-E-Y-U-A-N-Y-U,-†-C-H-R-I-S-T-O-P-H-E-R-A-M-R-H-2087974630;K. R. M. A T S U M O T O-K.-R.-M.-A-T-S-U-M-O-T-O-2087954552
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作者：
X. U E Y U A N Y U, † C H R I S T O P H E R A M R H E-X.-U-E-Y-U-A-N-Y-U,-†-C-H-R-I-S-T-O-P-H-E-R-A-M-R-H-2087974630;K. R. M. A T S U M O T O-K.-R.-M.-A-T-S-U-M-O-T-O-2087954552
对零价铁(ZVI)与高氯酸盐还原微生物(PRMs)联合去除地下水中高氯酸盐进行了一系列的间歇实验研究。在这种方法中，PRMS利用铁在水中腐蚀过程中产生的氢气作为电子供体，将高氯酸盐还原为氯化物。高氯酸盐的降解符合Monod动力学，归一化最大利用率为9200μg g-1(干质量)h-1，半速率常数Ks为8900μg L-1。高氯酸盐的总降解率受系统内生物质密度的影响。OD600值从0.025增加到0.08时，高氯酸盐的还原速率相应增加了4倍。在中性pH条件下，PRM对当地环境的适应和高氯酸盐还原的启动速度很快。在初始pH为7和8时，高氯酸盐还原遵循准一级反应速率，反应常数分别为0.059和0.033 h-1。一旦建立了高氯酸盐还原，生物还原过程对pH从近中性到9.0的增加不敏感。在硝酸盐存在下，高氯酸盐还原速率有所降低，但没有完全被抑制。
A series of batch experiments were performed to study the combination of zero-valent iron (ZVI) with perchloratereducing microorganisms (PRMs) to remove perchlorate from groundwater. In this method, H2 produced during the process of iron corrosion by water is used by PRMs as an electron donor to reduce perchlorate to chloride. Perchlorate degradation rates followed Monod kinetics, with a normalized maximum utilization rate (rmax) of 9200 μg g-1 (dry wt) h-1 and a half-velocity constant (Ks) of 8900 μg L-1. The overall rate of perchlorate reduction was affected by the biomass density within the system. An increase in the OD600 from 0.025 to 0.08 led to a corresponding 4-fold increase of perchlorate reduction rate. PRM adaptation to the local environment and initiation of perchlorate reduction was rapid under neutral pH conditions. At the initial OD600 of 0.015, perchlorate reduction followed pseudo-firstorder reaction rates with constants of 0.059 and 0.033 h-1 at initial pH 7 and 8, respectively. Once perchlorate reduction was established, the bioreductive process was insensitive to the increases of pH from near neutral to 9.0. In the presence of nitrate, perchlorate reduction rate was reduced, but not inhibited completely.