MOLECULAR-CLONING, FUNCTIONAL EXPRESSION AND MESSENGER-RNA ANALYSIS OF HUMAN BETA-ADRENERGIC-RECEPTOR KINASE-2
MOLECULAR-CLONING, FUNCTIONAL EXPRESSION AND MESSENGER-RNA ANALYSIS OF HUMAN BETA-ADRENERGIC-RECEPTOR KINASE-2
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DOI：
10.1006/bbrc.1993.1072
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发表时间：
1993-01-29
影响因子：
3.1
通讯作者：
DEBLASI, A
中科院分区：
文献类型：
--
作者：
PARRUTI, G;AMBROSINI, G;DEBLASI, A
本研究采用PCR和cDNA文库筛选相结合的方法克隆了人β ARK 2的cDNA，并将其亚克隆到表达载体中，在COS 7细胞中进行了瞬时表达。在体外磷酸化牛视杆细胞外节段中，表达的激酶活性是人β ARK 1的约40%。人和牛mRNA的北方印迹分析揭示了多个杂交条带的种属特异性模式，在人中有两个主要转录本，而不是牛中的一个。在外周血白细胞中发现高水平的mRNA表达。
In the present study the cDNA of human βARK2 was cloned using both PCR and cDNA library screening, subcloned into an expression vector and transiently expressed in COS7 cells. The expressed kinase activity was∼ 40% as efficient as human βARK1 in phosphorylating bovine rod outer segments in vitro. Northern blot analysis of human and bovine mRNA revealed a species-specific pattern of multiple hybridization bands, with two major transcripts in human rather than one in bovine. High levels of mRNA expression were found in peripheral blood leukocytes.