Tissue-cell- and species-specific expression of gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) in gonads, adrenal and brain. Identification of novel forms in the brain.
Tissue-cell- and species-specific expression of gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) in gonads, adrenal and brain. Identification of novel forms in the brain.
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促性腺激素调节的长链酰基辅酶A合成酶（GR-LACS）在性腺、肾上腺和大脑中的组织细胞和物种特异性表达。
DOI：
10.1016/j.jsbmb.2005.10.005
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Dufau,MariaL
中科院分区：
文献类型：
--
作者：
Li,Jie;Sheng,Yi;Tang,PeiZong;Tsai-Morris,Chon-Hwa;Dufau,MariaL
促性腺激素调节的长链酰基辅酶A合成酶（GR-LACS）是一种新的经尿道调节的脂肪酰基辅酶A合成酶（FACS），具有长链脂肪酸的活性。这种酶在成熟大鼠睾丸间质细胞中的存在及其调节模式表明，它参与睾丸类固醇生成。这项研究表明，GR-LACS的表达是组织，细胞和物种特异性。79 kDa GR-LACS蛋白在啮齿类动物的性腺和脑中表达，并且仅在小鼠的肾上腺皮质中表达。在这两个物种的卵巢中，它与卵泡闭锁有关。它存在于新生儿和未成熟的睾丸小管中，青春期后仅存在于Leydig细胞中。在大鼠脑中发现了一种独特的64 kDa的GR-LACS蛋白种类，其比79 kDa的长形式更丰富。此外，在大鼠脑和小鼠卵巢中观察到较小的73 kDa形式。在大鼠脑cDNA文库中鉴定了两种由GR-LACS基因的选择性剪接产生的新物种：缺少外显子8的短形式1（S1）和缺少外显子6-8的短形式2（S2）。表达研究显示，S1/S2蛋白的大小与内源性变体物种的大小相当。两种S型都不含FACS活性，表明外显子8对酶功能是必需的。GR-LACS变体对长型的FACS活性表现出小但显著的显性负效应。GR-LACS变体可以调节大脑中长形式的活动。
Gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) is a novel hormonally regulated fatty acyl-CoA synthetase (FACS) with activity for long-chain fatty acids. The presence of this enzyme in the Leydig cells of the mature rat testis and its mode of regulation suggest that it participates in testicular steroidogenesis. This study demonstrates that GR-LACS expression is tissue, cell and species-specific. The 79kDa GR-LACS protein is expressed in rodent gonads and brain, and only in the mouse in the adrenal cortex. In the ovary of both species it is associated with follicles undergoing atresia. It is present in the newborn and immature testis tubules and after puberty only in the Leydig cells. A distinct GR-LACS protein species of 64kDa that was more abundant than the 79kDa long form was found in the rat brain. Also, a minor 73kDa form was observed in the rat brain and mouse ovary. Two novel species resulting from alternatively splicing of the GR-LACS gene were identified in a rat brain cDNA library: a short form 1 (S1) lacking exon 8 and short form 2 (S2) lacking exons 6–8. Expression studies revealed that the sizes of the S1/S2 proteins are comparable to those of the endogenous variant species. Neither S form contains FACSs activity, suggesting that exon 8 is essential for the enzymatic function. GR-LACS variants exhibit small but significant dominant negative effects on the FACS activity of the long form. GR-LACS variants may regulate the long form's activity in the brain.