Regions in the 3′ untranslated region confer stage-specific expression to the Leishmania mexicana a600-4 gene
Regions in the 3′ untranslated region confer stage-specific expression to the Leishmania mexicana a600-4 gene
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DOI：
10.1016/j.molbiopara.2007.02.010
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发表时间：
2007-06-01
影响因子：
1.5
通讯作者：
McMaster, W. Robert
中科院分区：
文献类型：
--
作者：
Murray, Angus;Fu, Christine;McMaster, W. Robert
利什曼原虫属的原生动物寄生虫具有二发生的生命周期，在前鞭毛体和无鞭毛体阶段之间交替。细胞外前鞭毛体驻留在白蛉载体内，而专性细胞内无鞭毛体阶段在哺乳动物宿主巨噬细胞的吞噬溶酶体中复制。在这些截然不同的环境中适应和生存伴随着一部分基因的差异表达，这些基因通过Yun翻译区（3 'UTR）或顺反子间序列中的顺式作用元件进行转录后调节。先前报道墨西哥利什曼原虫A600-4 mRNA转录本丰度在无鞭毛体中高8倍。在这项研究中，嵌合荧光素酶：A600-4 3 'UTR报告基因构建体整合在A600染色体基因座上，以鉴定A600-4 3' UTR序列的调控区。证据提供了不同的3 'UTR元件的功能，以稳定A600-4 mRNA转录在无鞭毛体阶段和调节翻译效率，分别。(c)2007 Elsevier B. V.保留所有权利。
Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation to and survival within these vastly differently environments is accompanied by differential expression of a subset of genes, which is regulated post-transcriptionally via cis-acting elements in Yuntranslated region (3'UTR) or intercistronic sequences. It was reported previously that Leishmania mexicana A600-4 mRNA transcript abundance was eight-fold higher in the amastigotes. In this study, chimeric luciferase:A600-4 3UTR reporter constructs were integrated at the A600 chromosome locus to identify regulatory regions of the A600-4 3'UTR sequence. Evidence is provided for distinct 3'UTR elements that function to stabilize the A600-4 mRNA transcript in the amastigote stage and to regulate translation efficiency, respectively. (c) 2007 Elsevier B.V. All rights reserved.