MAPK cascade-mediated regulation of pathogenicity, conidiation and tolerance to abiotic stresses in the entomopathogenic fungus Metarhizium robertsii
MAPK cascade-mediated regulation of pathogenicity, conidiation and tolerance to abiotic stresses in the entomopathogenic fungus Metarhizium robertsii
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MAPK 级联介导的昆虫病原真菌绿僵菌致病性、分生孢子和对非生物胁迫耐受性的调节
DOI：
10.1111/1462-2920.13198
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Fang, Weiguo
中科院分区：
文献类型：
--
作者：
Chen, Xiaoxuan;Xu, Chuan;Fang, Weiguo
罗伯茨绿僵菌是研究真菌致病性的模式菌，其致病性与植物和哺乳动物病原真菌有许多相似之处。丝裂原活化蛋白激酶（MAPK）级联在真菌的细胞调控中起着关键作用，但它们的功能还没有在罗伯茨分枝杆菌的特点。在本研究中，我们确定了完整的丝裂原活化蛋白激酶级联组成部分，并剖析其在致病，分生孢子和胁迫耐受性的调节作用。Fus 3、Hog 1和Slt 2-MAPK级联的9个组分都参与分生孢子的形成。Fus 3-和Hog 1-MAPK级联是耐受高渗胁迫所必需的，Slt 2-和Fus 3-MAPK级联都介导细胞壁完整性。Hog 1和Slt 2-MAPK级联有助于致病性; Fus 3-MAPK级联对于真菌致病性是不可或缺的。在其生命周期中，罗氏沼虾经历了多种微环境，因为它穿过角质层进入血腔。RNA-seq分析表明，MAPK级联在调节罗氏沼虾对从角质层到血淋巴的微环境变化的适应中起主要作用。这三种MAPK在角质层渗透和血腔定殖过程中各自调节自己独特的基因子集，但它们在调节适应微环境变化方面具有冗余功能。
Metarhizium robertsii has been used as a model to study fungal pathogenesis in insects, and its pathogenicity has many parallels with plant and mammal pathogenic fungi. MAPK (Mitogen-activated protein kinase) cascades play pivotal roles in cellular regulation in fungi, but their functions have not been characterized in M.robertsii. In this study, we identified the full complement of MAPK cascade components in M.robertsii and dissected their regulatory roles in pathogenesis, conidiation and stress tolerance. The nine components of the Fus3, Hog1 and Slt2-MAPK cascades are all involved in conidiation. The Fus3- and Hog1-MAPK cascades are necessary for tolerance to hyperosmotic stress, and the Slt2- and Fus3-MAPK cascades both mediate cell wall integrity. The Hog1 and Slt2-MAPK cascades contribute to pathogenicity; the Fus3-MAPK cascade is indispensable for fungal pathogenesis. During its life cycle, M.robertsii experiences multiple microenvironments as it transverses the cuticle into the haemocoel. RNA-seq analysis revealed that MAPK cascades collectively play a major role in regulating the adaptation of M.robertsii to the microenvironmental change from the cuticle to the haemolymph. The three MAPKs each regulate their own distinctive subset of genes during penetration of the cuticle and haemocoel colonization, but they function redundantly to regulate adaptation to microenvironmental change.