Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein
Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein
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DOI：
10.1016/s0014-5793(98)00127-6
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发表时间：
1998-03-06
期刊：
影响因子：
3.5
通讯作者：
Nabeshima, Y
中科院分区：
文献类型：
--
作者：
Shiraki-Iida, T;Aizawa, H;Nabeshima, Y
我们先前建立了一种新的人类衰老小鼠模型，并确定了导致衰老的遗传基础。一种新基因klotho（kl）的表达缺陷导致小鼠出现类似人类衰老的综合征。kl基因编码单通道膜蛋白，其细胞外结构域携带与β-葡糖苷酶的同源性。在这份报告中，我们提出了整个小鼠kl基因组织。小鼠kl基因跨越约50个外显子，由5个外显子组成。启动子区缺乏TATA盒，并含有4个潜在的SP1结合位点。我们进一步表明，两个kl基因转录编码膜或分泌蛋白产生通过选择性转录终止。这些发现为进一步研究可能调控Five衰老的kl基因提供了基础信息。(C)1998年欧洲生物化学学会联合会。
We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed klotho (kl), leads to a syndrome resembling human aging in mice. The kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to beta-glucosidases. In this report, we present the entire mouse kl gene organization. The mouse kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the kl gene which may regulate aging in Five. (C) 1998 Federation of European Biochemical Societies.