Expression of Arabidopsis thaliana cytochrome P450 monooxygenase, CYP71A12, in yeast catalyzes the metabolism of herbicide pyrazoxyfen
Expression of Arabidopsis thaliana cytochrome P450 monooxygenase, CYP71A12, in yeast catalyzes the metabolism of herbicide pyrazoxyfen
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DOI：
10.5511/plantbiotechnology.24.393
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发表时间：
2007-09-01
影响因子：
1.6
通讯作者：
Imaishi, Hiromasa
中科院分区：
文献类型：
--
作者：
Hayashi, Etsuko;Fuzimoto, Kenji;Imaishi, Hiromasa
在高等植物中，细胞色素P450 （P450， CYP）单加氧酶在内源化合物的生物合成和代谢和/或除草剂、杀虫剂和杀菌剂等异种生物的氧化反应中起着重要作用。在本研究中，利用RT-PCR策略从野生型拟南芥生态型Ws中分离出编码CYP71A12的新型P450 cdna。Northern blot分析显示，cyp71A12在根中特异性表达，而在花、叶或茎中不表达。为了确定CYP71A12的功能，我们在酿酒酵母AH22细胞中表达了相应cdna的编码序列。表达CYP71A12的微粒体部分代谢除草剂吡唑氧芬。液相色谱-质谱（LC/MS）分析表明，吡唑氧芬代谢产物可能在吡唑环上发生n -去甲基化，在二氯苯环上发生5-羟基化。这是首次报道吡唑类除草剂被植物P450代谢。
In higher plants, cytochrome P450 (P450, CYP) monooxygenases play important roles in the biosynthesis and metabolism of endogenous compounds and/or in the oxidative reactions of xenobiotics, such as herbicides, insecticides, and fungicides. In this study, the novel P450 cDNAs encoding CYP71A12 was isolated from wild-type Arabidopsis thaliana ecotype Ws using an RT-PCR strategy. Northern blot analysis revealed that cyp71A12 is expressed specifically in roots but not in flowers, leaves, or stems. To determine the function of CYP71A12, the coding sequences of the corresponding cDNAs were expressed in Saccharomyces cerevisiae AH22 cells. Microsomal fractions expressing CYP71A12 metabolized the herbicide pyrazoxyfen. Liquid chromatography mass spectrometry (LC/MS) analysis indicated that the metabolite of pyrazoxyfen might be N-demethylated at the pyrazole ring and 5-hydroxylated at the dichlorobenzene ring. This is the first report that a pyrazole herbicide is metabolized by a plant P450 species.