Arabidopsis cryptochrome 1 is a soluble protein mediating blue light-dependent regulation of plant growth and development
Arabidopsis cryptochrome 1 is a soluble protein mediating blue light-dependent regulation of plant growth and development
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DOI：
10.1046/j.1365-313x.1996.10050893.x
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发表时间：
1996-11-01
期刊：
影响因子：
7.2
通讯作者：
Cashmore, AR
中科院分区：
文献类型：
--
作者：
Lin, CT;Ahmad, M;Cashmore, AR
隐花色素1(CRY1)是拟南芥中一种黄素型蓝光受体，介导抑制下胚轴伸长。在本报告中所描述的工作中，证明了CRY1是一种在黑暗或光照下生长的幼苗以及成年植物不同器官中表达的可溶性蛋白。利用高表达CRY1的转基因拟南芥进一步研究了CRY1的功能作用。结果表明，CRY1的过表达导致了对蓝光、UV-A光和绿光的超敏，从而抑制了下胚轴的伸长反应。过量表达CRY1的转基因植株也表现出矮化表型，几乎每个器官的大小都变小了。这与先前在hy4突变植株中发现的相互改变的观察结果一致，并与CRY1介导光依赖过程导致对植物生长产生普遍抑制效应的假说一致。此外，过量表达CRY1的转基因植株表现出对蓝光、UV-A和绿光的响应增加了花青素的积累，并且表现出依赖于注入率的方式。在转基因植株中，花青素积累的增加伴随着蓝光诱导CHS基因表达的增加。因此，CRY1除了对植物生长有影响外，还是一个光感受器，参与蓝光依赖的基因表达调控。
Cryptochrome 1 (CRY1) is a flavin-type blue light receptor of Arabidopsis thaliana which mediates inhibition of hypocotyl elongation. In the work described in this report it is demonstrated that CRY1 is a soluble protein expressed in both young seedlings grown either in the dark or under light, and in different organs of adult plants. The functional role of CRY1 was further investigated using transgenic Arabidopsis plants overexpressing CRY1. It is demonstrated that overexpression of CRY1 resulted in hypersensitivity to blue, UV-A, and green light for the inhibition of hypocotyl elongation response. Transgenic plants overexpressing CRY1 also exhibited a dwarf phenotype with reduced size in almost every organ. This was in keeping with the previous observation of reciprocal alterations found in hy4 mutant plants and is consistent with a hypothesis that CRY1 mediates a light-dependent process resulting in a general inhibitory effect on plant growth. In addition, transgenic plants overexpressing CRY1 showed increased anthocyanin accumulation in response to blue, UV-A, and green light in a fluence rate-dependent manner. This increase in anthocyanin accumulation in transgenic plants was shown to be concomitant with increased blue light-induction of CHS gene expression. It is concluded that CRY1 is a photoreceptor mediating blue light-dependent regulation of gene expression in addition to its affect on plant growth.